FastQCFastQC Report
Fri 22 Mar 2024
20.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16033456
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGG3111631.9407107238763746TruSeq Adapter, Index 1 (97% over 36bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT271870.1695641912760418No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATATGGGGG248240.15482625829390742TruSeq Adapter, Index 1 (97% over 36bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG216920.1352921042100967No Hit
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA163130.10174350433244087No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC160470.10008447336619129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)