FastQCFastQC Report
Fri 22 Mar 2024
2.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18269876
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1469450.8043021200581766No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT337870.1849328369825827No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA332430.1819552579338798No Hit
CTCCACCAGACCAACACCATGAAATTCGCCGCTCTTGCTCTTGCCCTTCT230230.12601618095273334No Hit
TGAAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGAC201920.11052072821950186No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC188200.1030110987069644No Hit
CTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAAAATTC183260.10030719420317903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)