FastQCFastQC Report
Fri 22 Mar 2024
147.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename147.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8149461
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG867541.064536660768117TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168520.206786681965838No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGT127400.1563293572421538TruSeq Adapter, Index 7 (97% over 38bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT102310.12554204505058678No Hit
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC89050.10927103031721977No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC84080.10317246747975112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)