FastQCFastQC Report
Fri 22 Mar 2024
139.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename139.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9025836
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG509830.5648562637300302TruSeq Adapter, Index 7 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG193370.21424054237191992No Hit
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT132200.14646842685818798No Hit
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA127080.1407958221266152No Hit
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC102000.11300892238680163No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC90320.1000682928429012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)