FastQCFastQC Report
Fri 22 Mar 2024
138.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename138.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7914394
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1460181.844967536364755No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT165120.2086325244863978No Hit
CATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAA153400.19382406284043982No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA130890.16538221372350176No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA125270.15828122784890417No Hit
GGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAAGAA110290.13935368898743225No Hit
GGAGAAGACATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAG94310.11916262950770458No Hit
TGTGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAA81310.10273686147037916No Hit
GAATACTGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGA80670.10192820827469545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)