FastQCFastQC Report
Fri 22 Mar 2024
138.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename138.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7914394
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG732080.9249981742127066TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGT110130.13915152568851133TruSeq Adapter, Index 7 (97% over 38bp)
CAAAAATGAAGCCATAGCAGTAATCAATAATACCTTCTAGAGTCTGACTA104230.1316967540408021No Hit
GTCGTATGCAAGTGGTTTAACACCCAGTCCTTCATAAACATGTGGTGTGA94400.1192763463633476No Hit
TTTTGTTATTAACATCACCAAGTCAACCCAACGAAATTAACCGGATACAA83120.10502383378942215No Hit
GTCCATTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGC81150.10253469817145823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)