FastQCFastQC Report
Fri 22 Mar 2024
131.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename131.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8424948
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1778482.1109685187374447No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA164950.19578755857009444No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA126810.15051724948332026No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG116890.13874269609735276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)