FastQCFastQC Report
Fri 22 Mar 2024
131.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename131.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8424948
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG950861.1286241766714762TruSeq Adapter, Index 7 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG161380.19155014369228154No Hit
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA137930.16371614400468704No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGT136080.1615202847542798TruSeq Adapter, Index 7 (97% over 38bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT86370.1025169532203641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)