FastQCFastQC Report
Fri 22 Mar 2024
129.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8824155
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG99880111.31894215366797TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGT1448871.6419362533863016TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGATGAGATCTGGGGG876580.9933869022019672TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGGAGATCTGGGGG449380.5092612267123594TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGAG360510.40854903387349834TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATATGGGGG331630.37582068764657917TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGTGG203700.2308436331864071TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168780.19127043892588014No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCGGGGGG167880.19025051123875317TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTAGGGG130140.1474815435585617TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGTGT127530.14452375326589345TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGATGAGATCTGGGGT125400.14210992440635958TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTGATGAGATCTGGGGG113900.12907751507084814TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGGAGATCTGGGGG106020.12014748154355857TruSeq Adapter, Index 1 (97% over 37bp)
GTCCATTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGC93030.10542652525935912No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)