FastQCFastQC Report
Fri 22 Mar 2024
128.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename128.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9343373
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG491720.526276752517533TruSeq Adapter, Index 7 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG131800.14106254775443514No Hit
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT131110.14032405641945367No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108130.11572908413267885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)