FastQCFastQC Report
Fri 22 Mar 2024
121.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename121.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19933243
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATCTGGGGG2040791.0238123319923405TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATCTAGGGG411480.2064290291348979TruSeq Adapter, Index 7 (97% over 36bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT292280.14662942703302217No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATATGGGGG207110.10390180865200911TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)