FastQCFastQC Report
Fri 22 Mar 2024
118.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename118.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19651360
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2074731.0557691681389991No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA262470.13356327500997386No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT259710.13215879206324652No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA221080.11250111951539232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)