FastQCFastQC Report
Fri 22 Mar 2024
110.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename110.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19839132
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1849580.9322887715047211No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA479660.2417746905459372No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT443340.22346743799073468No Hit
CTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAAAATTC267660.13491517673253042No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC266090.1341238114651387No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA256270.129173998136612No Hit
GTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAAA249740.12588252348943493No Hit
TGAAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGAC210140.10592197279598724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)