FastQCFastQC Report
Fri 22 Mar 2024
109.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename109.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20663384
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4494802.175248739509463No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT358060.17328236265657165No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA340960.1650068546371688No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA300010.14518919069596734No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC244740.1184413937233127No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA223320.10807523104637654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)