FastQCFastQC Report
Fri 22 Mar 2024
10.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename10.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18424782
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTGGGGG1393760.756459425137296TruSeq Adapter, Index 9 (97% over 36bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT340620.18487057268845838No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG270020.14655261592783025No Hit
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA247910.13455247394514627No Hit
ATGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTC199720.10839748334607162No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC196170.10647073056278224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)