FastQCFastQC Report
Mon 4 Mar 2024
90_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename90_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18289278
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3485761.9059035572645349No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA766900.41931671660302827No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC279920.15305142171276526No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263450.14404614550667338No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA218410.1194196949710098No Hit
GGGACTCAATAGCTTTCGATCTCACACACACTCACACACACACACACACA212320.11608987517167163No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA203950.11151342332923148No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA193850.10599106208566572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)