FastQCFastQC Report
Mon 4 Mar 2024
88_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename88_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20182056
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4562142.260493182656911No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA969570.4804119064975343No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324790.16093008561664876No Hit
GGGACTCAATAGCTTTCGATCTCACACACACTCACACACACACACACACA255840.126766073783563No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC237910.11788194423799042No Hit
CACACACTCACACACACACACACACACACACACACACACACACACACACA218240.10813566268966848No Hit
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT209030.10357220295097783No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA208970.10354247357157269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)