FastQCFastQC Report
Mon 4 Mar 2024
57-S_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename57-S_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13188766
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG4212173.1937559586696738TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG3687622.796031107080071TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATATGGGGG509390.38623022047703326TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATATAGGGG441030.3343982295235202TruSeq Adapter, Index 27 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294070.22297006406816225No Hit
CTCAGATGCAACTGTAAAACCTTGGGGTGCTTATTTTTAAAACTAGCCAA276740.20983009327786997No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGG271140.20558405539987593TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCGGGGGG228650.1733672430005961TruSeq Adapter, Index 27 (97% over 38bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA222540.16873451238728476No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGG221860.16821892207352834TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCGAGGGG191040.14485054932356828TruSeq Adapter, Index 27 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185720.1408168133394739No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGT177800.13481170262631093TruSeq Adapter, Index 27 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG172400.1307173089582452No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGT160980.12205842457133594TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)