FastQCFastQC Report
Mon 4 Mar 2024
38_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename38_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18364073
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG5164202.812121254364432TruSeq Adapter, Index 2 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT349040.19006676786789076No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATATGGGGG314860.17145433913271854TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTAGGGG276330.15047315483879856TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249370.1357923157896399No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC235970.12849545958568123No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCGGGGGG216160.11770809231699307TruSeq Adapter, Index 2 (97% over 37bp)
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC200220.10902810068332881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)