Version MEGAN Community Edition (version 6.21.5, built 5 May 2021) Author(s) Daniel H. Huson Copyright (C) 2021 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,302,807 Loading ncbi.tre: 2,302,811 Loading ec.map: 8,081 Loading ec.tre: 8,085 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 13,894 Loading interpro2go.tre: 28,869 Loading seed.map: 979 Loading seed.tre: 980 In DAA file: Trinity.fasta.blastx.daa Output file: Trinity.fasta.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file Trinity.fasta.blastx.daa Parsing file: Trinity.fasta.blastx.daa Input domination filter: MinPercentCoverToStronglyDominate=90.0 and TopPercentScoreToStronglyDominate=90.0 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (946.4s) Total reads: 331,509 Alignments: 7,648,952 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Interval-Union-LCA' algorithm (51.0 %) for binning: Taxonomy Using Multi-Gene Best-Hit algorithm for binning: SEED Using Multi-Gene Best-Hit algorithm for binning: EGGNOG Using 'Interval-Union-LCA' algorithm (51.0 %) for binning: GTDB Using Multi-Gene Best-Hit algorithm for binning: EC Using Multi-Gene Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 331,509 Total weight: 199,956,726 With hits: 331,509 Alignments: 7,648,952 Assig. Taxonomy: 330,980 Assig. SEED: 1,173 Assig. EGGNOG: 988 Assig. GTDB: 6,445 Assig. EC: 47,871 Assig. INTERPRO2GO: 19,001 MinSupport set to: 99978 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 1,361 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 966 Binning reads: Writing classification tables Numb. Tax. classes: 100 Numb. SEED classes: 214 Numb. EGG. classes: 399 Numb. GTDB classes: 18 Numb. EC classes: 1,358 Numb. INT. classes: 3,604 Binning reads: Syncing Class. Taxonomy: 100 Class. SEED: 214 Class. EGGNOG: 399 Class. GTDB: 18 Class. EC: 1,358 Class. INTERPRO2GO: 3,604 100% (6854.3s) Total time: 7,810s Peak memory: 105.9 of 488.3G --------------------- Total runtime was: 7812 seconds