## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[P15-female_nonbleached_K5.sorted.bam] OUTPUT=P15-female_nonbleached_K5.sorted.dedup.bam METRICS_FILE=P15-female_nonbleached_K5-picard_metrics.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Jul 18 20:30:25 PDT 2019 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 1027436 32697079 0 2642852 668774 12332068 855972 0.381396 32758302 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.015696 13805859 0 14067280 2.0 1.390048 4023429 672272 3977937 3.0 1.528022 1406942 67372 1331173 4.0 1.578875 559531 10768 503402 5.0 1.597618 247574 2326 212040 6.0 1.604526 118721 640 98445 7.0 1.607072 62968 216 51557 8.0 1.60801 36627 124 30359 9.0 1.608356 22700 65 19212 10.0 1.608484 15442 46 13328 11.0 1.608531 10956 22 9715 12.0 1.608548 8260 24 7598 13.0 1.608554 6377 7 5780 14.0 1.608557 5083 8 4630 15.0 1.608558 4141 4 3897 16.0 1.608558 3410 9 3241 17.0 1.608558 2964 2 2779 18.0 1.608558 2431 3 2277 19.0 1.608558 2131 1 1996 20.0 1.608558 1796 1 1698 21.0 1.608558 1585 0 1455 22.0 1.608558 1325 0 1323 23.0 1.608558 1248 2 1184 24.0 1.608558 1100 1 982 25.0 1.608558 910 0 882 26.0 1.608558 854 0 788 27.0 1.608558 734 0 712 28.0 1.608558 680 0 635 29.0 1.608558 616 0 573 30.0 1.608558 560 0 524 31.0 1.608558 500 0 467 32.0 1.608558 454 0 431 33.0 1.608558 403 0 404 34.0 1.608558 377 0 334 35.0 1.608558 355 0 350 36.0 1.608558 316 0 285 37.0 1.608558 309 0 296 38.0 1.608558 255 0 277 39.0 1.608558 284 0 270 40.0 1.608558 268 0 232 41.0 1.608558 229 0 203 42.0 1.608558 195 0 187 43.0 1.608558 205 0 192 44.0 1.608558 185 0 181 45.0 1.608558 183 0 162 46.0 1.608558 157 0 151 47.0 1.608558 141 0 130 48.0 1.608558 126 0 122 49.0 1.608558 123 0 130 50.0 1.608558 117 0 117 51.0 1.608558 143 0 124 52.0 1.608558 119 0 110 53.0 1.608558 88 0 101 54.0 1.608558 108 0 104 55.0 1.608558 103 0 87 56.0 1.608558 84 0 65 57.0 1.608558 89 0 86 58.0 1.608558 73 0 76 59.0 1.608558 72 0 58 60.0 1.608558 68 0 71 61.0 1.608558 70 0 62 62.0 1.608558 59 0 66 63.0 1.608558 66 0 57 64.0 1.608558 54 0 56 65.0 1.608558 68 0 51 66.0 1.608558 61 0 62 67.0 1.608558 44 0 48 68.0 1.608558 48 0 43 69.0 1.608558 54 0 42 70.0 1.608558 33 0 31 71.0 1.608558 39 0 42 72.0 1.608558 47 0 49 73.0 1.608558 41 0 30 74.0 1.608558 35 0 38 75.0 1.608558 33 0 27 76.0 1.608558 40 0 32 77.0 1.608558 23 0 37 78.0 1.608558 38 0 31 79.0 1.608558 24 0 23 80.0 1.608558 31 0 17 81.0 1.608558 30 0 23 82.0 1.608558 21 0 26 83.0 1.608558 22 0 25 84.0 1.608558 21 0 24 85.0 1.608558 28 0 22 86.0 1.608558 25 0 23 87.0 1.608558 25 0 22 88.0 1.608558 14 0 20 89.0 1.608558 19 0 25 90.0 1.608558 26 0 19 91.0 1.608558 18 0 18 92.0 1.608558 27 0 19 93.0 1.608558 17 0 18 94.0 1.608558 25 0 17 95.0 1.608558 14 0 20 96.0 1.608558 16 0 14 97.0 1.608558 19 0 10 98.0 1.608558 17 0 15 99.0 1.608558 12 0 11 100.0 1.608558 12 0 11 101.0 0 12 0 17 102.0 0 9 0 10 103.0 0 7 0 14 104.0 0 14 0 12 105.0 0 15 0 13 106.0 0 15 0 18 107.0 0 9 0 9 108.0 0 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