## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[P14-female_bleached_44.sorted.bam] OUTPUT=P14-female_bleached_44.sorted.dedup.bam METRICS_FILE=P14-female_bleached_44-picard_metrics.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Jul 18 19:33:32 PDT 2019 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 1307608 29718261 0 2569598 712426 7235381 1215530 0.249953 57597911 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.032616 17731188 0 18342662 2.0 1.649012 3379780 941696 3086576 3.0 2.016955 878500 102453 703946 4.0 2.23659 280347 16335 200845 5.0 2.367696 104265 3338 70018 6.0 2.445957 45083 799 30062 7.0 2.492673 21975 223 15177 8.0 2.520559 12131 75 8839 9.0 2.537205 7331 22 5663 10.0 2.547141 4868 16 3891 11.0 2.553073 3366 5 2905 12.0 2.556613 2428 6 2098 13.0 2.558727 1938 5 1702 14.0 2.559988 1432 3 1266 15.0 2.560741 1216 0 1040 16.0 2.561191 1014 1 871 17.0 2.561459 753 3 674 18.0 2.561619 654 0 566 19.0 2.561715 526 1 457 20.0 2.561772 425 4 373 21.0 2.561806 402 0 358 22.0 2.561826 333 1 292 23.0 2.561838 289 0 233 24.0 2.561846 259 0 218 25.0 2.56185 205 0 215 26.0 2.561853 173 0 177 27.0 2.561854 175 0 149 28.0 2.561855 141 0 136 29.0 2.561856 131 0 106 30.0 2.561856 133 0 115 31.0 2.561856 102 0 88 32.0 2.561856 93 0 90 33.0 2.561856 92 0 83 34.0 2.561856 90 0 74 35.0 2.561856 86 0 58 36.0 2.561856 63 0 68 37.0 2.561856 67 0 56 38.0 2.561856 54 0 45 39.0 2.561856 43 0 45 40.0 2.561856 43 0 49 41.0 2.561856 47 0 39 42.0 2.561856 38 0 32 43.0 2.561856 39 0 32 44.0 2.561856 31 0 36 45.0 2.561856 32 0 30 46.0 2.561856 34 0 27 47.0 2.561856 30 0 32 48.0 2.561856 30 0 24 49.0 2.561856 27 0 17 50.0 2.561856 25 0 20 51.0 2.561856 15 0 16 52.0 2.561856 17 0 15 53.0 2.561856 15 0 23 54.0 2.561856 20 0 14 55.0 2.561856 12 0 5 56.0 2.561856 14 0 12 57.0 2.561856 16 0 9 58.0 2.561856 13 0 13 59.0 2.561856 13 0 7 60.0 2.561856 12 0 14 61.0 2.561856 13 0 9 62.0 2.561856 6 0 3 63.0 2.561856 6 0 5 64.0 2.561856 8 0 6 65.0 2.561856 8 0 6 66.0 2.561856 6 0 5 67.0 2.561856 2 0 6 68.0 2.561856 6 0 3 69.0 2.561856 2 0 2 70.0 2.561856 6 0 4 71.0 2.561856 4 0 7 72.0 2.561856 5 0 3 73.0 2.561856 3 0 4 74.0 2.561856 8 0 5 75.0 2.561856 7 0 3 76.0 2.561856 3 0 3 77.0 2.561856 6 0 5 78.0 2.561856 1 0 6 79.0 2.561856 7 0 2 80.0 2.561856 1 0 5 81.0 2.561856 1 0 4 82.0 2.561856 3 0 3 83.0 2.561856 5 0 0 84.0 2.561856 3 0 5 85.0 2.561856 4 0 3 86.0 2.561856 0 0 0 87.0 2.561856 3 0 2 88.0 2.561856 4 0 3 89.0 2.561856 1 0 1 90.0 2.561856 2 0 0 91.0 2.561856 2 0 2 92.0 2.561856 2 0 0 93.0 2.561856 1 0 1 94.0 2.561856 5 0 5 95.0 2.561856 2 0 1 96.0 2.561856 1 0 2 97.0 2.561856 1 0 2 98.0 2.561856 2 0 1 99.0 2.561856 1 0 0 100.0 2.561856 0 0 1 101.0 0 1 0 1 103.0 0 0 0 1 104.0 0 3 0 0 105.0 0 1 0 0 108.0 0 1 0 1 109.0 0 0 0 2 110.0 0 2 0 1 112.0 0 1 0 1 113.0 0 1 0 1 114.0 0 3 0 1 115.0 0 1 0 1 116.0 0 1 0 1 118.0 0 0 0 1 119.0 0 0 0 1 120.0 0 0 0 1 121.0 0 0 0 1 122.0 0 1 0 0 123.0 0 2 0 0 124.0 0 1 0 1 125.0 0 1 0 0 126.0 0 1 0 1 130.0 0 1 0 1 132.0 0 0 0 1 135.0 0 1 0 0 136.0 0 1 0 0 139.0 0 0 0 1 141.0 0 1 0 0 142.0 0 0 0 1 144.0 0 1 0 1 146.0 0 0 0 1 149.0 0 1 0 0 150.0 0 0 0 1 153.0 0 1 0 0 155.0 0 1 0 0 156.0 0 1 0 0 158.0 0 0 0 1 162.0 0 1 0 1 163.0 0 0 0 1 166.0 0 0 0 1 169.0 0 1 0 0 173.0 0 1 0 1 178.0 0 1 0 0 182.0 0 1 0 0 183.0 0 0 0 1 186.0 0 1 0 0 193.0 0 0 0 2 195.0 0 1 0 0 198.0 0 1 0 0 206.0 0 0 0 1 212.0 0 0 0 1 217.0 0 1 0 0 219.0 0 1 0 0 224.0 0 0 0 1 235.0 0 0 0 1 237.0 0 0 0 1 238.0 0 0 0 1 239.0 0 0 0 1 240.0 0 1 0 0 241.0 0 1 0 0 247.0 0 0 0 1 249.0 0 1 0 0 250.0 0 0 0 1 251.0 0 1 0 0 253.0 0 1 0 0 255.0 0 0 0 1 256.0 0 0 0 1 258.0 0 1 0 0 259.0 0 0 0 1 262.0 0 1 0 0 265.0 0 1 0 0 267.0 0 1 0 0 277.0 0 1 0 0 282.0 0 0 0 1 287.0 0 0 0 2 290.0 0 1 0 0 296.0 0 0 0 1 305.0 0 1 0 0 306.0 0 1 0 0 315.0 0 1 0 0 443.0 0 0 0 1 448.0 0 1 0 0