## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[P11-female_bleached_44.sorted.bam] OUTPUT=P11-female_bleached_44.sorted.dedup.bam METRICS_FILE=P11-female_bleached_44-picard_metrics.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Jul 18 17:34:00 PDT 2019 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 823092 20668023 0 1719198 455804 5060495 732637 0.250878 38995912 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.027891 12454154 0 12824521 2.0 1.63291 2237434 576944 2054681 3.0 1.989025 567809 58215 463492 4.0 2.198635 183311 8946 137045 5.0 2.322012 71104 1899 51624 6.0 2.394632 32743 468 24268 7.0 2.437376 17626 147 13651 8.0 2.462536 10298 52 8546 9.0 2.477345 6858 34 5869 10.0 2.486061 4766 20 4221 11.0 2.491192 3490 6 3091 12.0 2.494212 2749 9 2509 13.0 2.495989 2168 5 1940 14.0 2.497035 1767 13 1622 15.0 2.497651 1368 5 1288 16.0 2.498014 1126 0 1029 17.0 2.498227 959 2 889 18.0 2.498353 848 5 800 19.0 2.498426 707 1 633 20.0 2.49847 565 0 535 21.0 2.498496 518 0 477 22.0 2.498511 440 0 408 23.0 2.49852 402 2 380 24.0 2.498525 349 1 337 25.0 2.498528 291 0 299 26.0 2.49853 293 1 248 27.0 2.498531 252 1 254 28.0 2.498531 232 0 180 29.0 2.498532 183 0 205 30.0 2.498532 213 1 183 31.0 2.498532 164 0 154 32.0 2.498532 164 0 143 33.0 2.498532 144 0 132 34.0 2.498532 126 0 101 35.0 2.498532 117 0 101 36.0 2.498532 97 0 91 37.0 2.498532 103 0 114 38.0 2.498532 83 0 80 39.0 2.498532 85 0 64 40.0 2.498532 77 0 62 41.0 2.498532 67 0 69 42.0 2.498532 69 0 59 43.0 2.498532 53 0 48 44.0 2.498532 44 0 51 45.0 2.498532 57 0 57 46.0 2.498532 57 0 42 47.0 2.498532 42 0 42 48.0 2.498532 34 0 29 49.0 2.498532 33 0 29 50.0 2.498532 37 0 36 51.0 2.498532 30 0 28 52.0 2.498532 32 1 35 53.0 2.498532 27 0 28 54.0 2.498532 28 0 23 55.0 2.498532 28 0 32 56.0 2.498532 29 0 34 57.0 2.498532 24 0 25 58.0 2.498532 25 0 17 59.0 2.498532 35 0 33 60.0 2.498532 23 0 24 61.0 2.498532 24 0 17 62.0 2.498532 24 0 16 63.0 2.498532 10 0 8 64.0 2.498532 15 0 19 65.0 2.498532 26 0 23 66.0 2.498532 16 0 18 67.0 2.498532 20 0 22 68.0 2.498532 19 0 9 69.0 2.498532 13 0 9 70.0 2.498532 13 0 15 71.0 2.498532 10 0 14 72.0 2.498532 7 0 10 73.0 2.498532 9 0 13 74.0 2.498532 14 0 9 75.0 2.498532 6 0 5 76.0 2.498532 7 0 9 77.0 2.498532 13 0 7 78.0 2.498532 9 0 7 79.0 2.498532 5 0 6 80.0 2.498532 10 0 13 81.0 2.498532 13 0 8 82.0 2.498532 10 0 6 83.0 2.498532 8 0 11 84.0 2.498532 9 0 0 85.0 2.498532 6 0 6 86.0 2.498532 4 0 9 87.0 2.498532 6 0 4 88.0 2.498532 6 0 5 89.0 2.498532 7 0 1 90.0 2.498532 2 0 7 91.0 2.498532 3 0 8 92.0 2.498532 3 0 8 93.0 2.498532 7 0 3 94.0 2.498532 3 0 4 95.0 2.498532 5 0 3 96.0 2.498532 7 0 7 97.0 2.498532 4 0 3 98.0 2.498532 6 0 7 99.0 2.498532 5 0 4 100.0 2.498532 8 0 5 101.0 0 2 0 2 102.0 0 6 0 4 103.0 0 4 0 2 104.0 0 3 0 3 105.0 0 6 0 2 106.0 0 2 0 3 107.0 0 4 0 2 108.0 0 1 0 1 109.0 0 2 0 2 110.0 0 2 0 1 111.0 0 3 0 3 112.0 0 0 0 4 113.0 0 3 0 5 114.0 0 1 0 1 115.0 0 4 0 3 116.0 0 0 0 1 117.0 0 1 0 3 118.0 0 4 0 1 119.0 0 4 0 2 120.0 0 3 0 2 121.0 0 3 0 2 122.0 0 1 0 0 123.0 0 3 0 1 124.0 0 1 0 1 125.0 0 1 0 3 126.0 0 0 0 3 127.0 0 1 0 2 128.0 0 3 0 1 129.0 0 1 0 1 131.0 0 0 0 4 132.0 0 6 0 6 133.0 0 4 0 4 134.0 0 3 0 1 135.0 0 2 0 2 136.0 0 1 0 0 137.0 0 4 0 1 138.0 0 2 0 1 139.0 0 0 0 2 140.0 0 0 0 2 141.0 0 2 0 2 142.0 0 1 0 1 143.0 0 2 0 1 144.0 0 3 0 1 145.0 0 3 0 2 146.0 0 2 0 1 147.0 0 0 0 3 149.0 0 1 0 1 150.0 0 1 0 1 151.0 0 0 0 1 152.0 0 4 0 1 153.0 0 2 0 2 154.0 0 1 0 0 155.0 0 2 0 0 156.0 0 0 0 2 157.0 0 4 0 0 159.0 0 0 0 3 161.0 0 1 0 2 163.0 0 1 0 0 164.0 0 2 0 0 165.0 0 0 0 1 167.0 0 1 0 1 168.0 0 1 0 2 169.0 0 0 0 1 170.0 0 0 0 1 172.0 0 3 0 1 176.0 0 1 0 2 177.0 0 1 0 1 179.0 0 2 0 0 180.0 0 1 0 2 182.0 0 0 0 2 183.0 0 2 0 1 185.0 0 1 0 2 186.0 0 1 0 1 187.0 0 2 0 0 188.0 0 0 0 2 191.0 0 1 0 0 193.0 0 1 0 1 194.0 0 1 0 0 196.0 0 1 0 0 199.0 0 2 0 1 200.0 0 0 0 1 203.0 0 0 0 1 205.0 0 0 0 1 209.0 0 0 0 1 211.0 0 1 0 0 212.0 0 2 0 0 213.0 0 2 0 0 214.0 0 1 0 0 218.0 0 0 0 1 221.0 0 1 0 0 222.0 0 0 0 1 231.0 0 0 0 1 233.0 0 1 0 1 235.0 0 1 0 0 237.0 0 1 0 0 239.0 0 0 0 1 242.0 0 1 0 0 243.0 0 0 0 1 246.0 0 0 0 1 247.0 0 1 0 0 251.0 0 1 0 0 255.0 0 0 0 1 257.0 0 1 0 1 261.0 0 0 0 1 264.0 0 0 0 1 272.0 0 1 0 0 274.0 0 1 0 0 275.0 0 0 0 1 277.0 0 1 0 0 278.0 0 1 0 0 295.0 0 0 0 1 299.0 0 0 0 1 301.0 0 0 0 1 302.0 0 0 0 1 304.0 0 1 0 0 305.0 0 0 0 1 312.0 0 1 0 0 314.0 0 1 0 0 317.0 0 1 0 0 319.0 0 0 0 1 320.0 0 0 0 1 330.0 0 1 0 0 331.0 0 1 0 0 332.0 0 1 0 0 352.0 0 0 0 1 361.0 0 1 0 0 373.0 0 0 0 1 379.0 0 0 0 1 390.0 0 1 0 0 430.0 0 1 0 0 455.0 0 0 0 1 459.0 0 0 0 1 473.0 0 1 0 0 475.0 0 1 0 0 504.0 0 0 0 1 527.0 0 1 0 0 531.0 0 0 0 1 545.0 0 1 0 0 610.0 0 0 0 1 613.0 0 0 0 1 635.0 0 2 0 0