## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[P10-female_nonbleached_K5.sorted.bam] OUTPUT=P10-female_nonbleached_K5.sorted.dedup.bam METRICS_FILE=P10-female_nonbleached_K5-picard_metrics.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Jul 18 16:49:16 PDT 2019 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 1338765 31035040 0 2706575 728334 7675908 1272320 0.253595 58841413 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.032492 18351545 0 18974099 2.0 1.641783 3540253 976019 3254532 3.0 2.001336 936770 109051 756546 4.0 2.213514 301646 18265 216462 5.0 2.338723 113356 3737 75502 6.0 2.412611 48628 995 31589 7.0 2.456214 23272 268 15639 8.0 2.481945 12584 89 8932 9.0 2.497129 7740 29 5925 10.0 2.506089 5113 18 3993 11.0 2.511377 3534 16 2935 12.0 2.514497 2503 6 2134 13.0 2.516339 1956 5 1725 14.0 2.517425 1453 2 1315 15.0 2.518067 1258 2 1048 16.0 2.518445 952 1 823 17.0 2.518668 770 1 721 18.0 2.5188 681 2 652 19.0 2.518878 610 2 477 20.0 2.518924 483 0 442 21.0 2.518951 415 1 363 22.0 2.518967 345 0 332 23.0 2.518976 295 3 295 24.0 2.518982 260 0 221 25.0 2.518985 238 0 212 26.0 2.518987 180 0 155 27.0 2.518988 162 1 160 28.0 2.518989 176 1 140 29.0 2.518989 147 0 137 30.0 2.518989 141 0 119 31.0 2.51899 122 0 112 32.0 2.51899 117 1 91 33.0 2.51899 87 0 89 34.0 2.51899 93 0 89 35.0 2.51899 76 0 73 36.0 2.51899 77 0 76 37.0 2.51899 73 0 59 38.0 2.51899 53 1 51 39.0 2.51899 62 0 49 40.0 2.51899 51 0 48 41.0 2.51899 38 0 35 42.0 2.51899 31 0 33 43.0 2.51899 55 0 31 44.0 2.51899 29 0 36 45.0 2.51899 35 0 41 46.0 2.51899 32 1 28 47.0 2.51899 31 0 22 48.0 2.51899 36 0 26 49.0 2.51899 29 0 20 50.0 2.51899 16 0 17 51.0 2.51899 19 1 19 52.0 2.51899 17 0 25 53.0 2.51899 17 0 16 54.0 2.51899 20 1 24 55.0 2.51899 24 0 13 56.0 2.51899 22 0 23 57.0 2.51899 21 0 15 58.0 2.51899 13 0 21 59.0 2.51899 16 0 15 60.0 2.51899 21 0 11 61.0 2.51899 12 1 9 62.0 2.51899 14 0 17 63.0 2.51899 11 0 11 64.0 2.51899 13 0 7 65.0 2.51899 10 0 10 66.0 2.51899 12 0 8 67.0 2.51899 9 0 9 68.0 2.51899 7 0 12 69.0 2.51899 11 0 6 70.0 2.51899 8 0 5 71.0 2.51899 4 0 7 72.0 2.51899 9 0 10 73.0 2.51899 4 0 7 74.0 2.51899 6 0 4 75.0 2.51899 6 0 4 76.0 2.51899 7 0 2 77.0 2.51899 4 0 6 78.0 2.51899 9 0 4 79.0 2.51899 1 0 5 80.0 2.51899 3 0 8 81.0 2.51899 7 0 6 82.0 2.51899 6 0 0 83.0 2.51899 4 0 2 84.0 2.51899 3 0 3 85.0 2.51899 3 0 1 86.0 2.51899 5 0 6 87.0 2.51899 2 0 7 88.0 2.51899 5 0 7 89.0 2.51899 5 0 6 90.0 2.51899 4 0 1 91.0 2.51899 5 0 3 92.0 2.51899 3 0 1 93.0 2.51899 5 0 2 94.0 2.51899 2 0 4 95.0 2.51899 3 0 5 96.0 2.51899 1 0 3 97.0 2.51899 1 0 5 98.0 2.51899 8 0 2 99.0 2.51899 4 0 2 100.0 2.51899 3 0 4 101.0 0 2 0 2 102.0 0 3 0 1 103.0 0 2 0 3 104.0 0 2 0 1 105.0 0 5 0 0 106.0 0 4 0 2 107.0 0 3 0 3 108.0 0 0 0 2 109.0 0 1 0 1 110.0 0 0 0 1 111.0 0 2 0 1 112.0 0 2 0 1 113.0 0 1 0 1 114.0 0 1 0 2 116.0 0 1 0 0 117.0 0 1 0 1 118.0 0 3 0 1 119.0 0 1 0 1 120.0 0 1 0 1 121.0 0 1 0 1 122.0 0 1 0 0 123.0 0 1 0 2 124.0 0 1 0 0 125.0 0 2 0 2 126.0 0 1 0 2 127.0 0 1 0 0 129.0 0 2 0 1 130.0 0 1 0 0 131.0 0 1 0 0 132.0 0 0 0 3 133.0 0 1 0 1 136.0 0 0 0 1 137.0 0 0 0 1 138.0 0 1 0 1 139.0 0 3 0 0 140.0 0 0 0 2 141.0 0 1 0 0 142.0 0 2 0 0 143.0 0 1 0 0 144.0 0 1 0 0 146.0 0 0 0 1 148.0 0 2 0 1 150.0 0 1 0 1 152.0 0 0 0 1 155.0 0 0 0 1 156.0 0 2 0 0 163.0 0 0 0 1 167.0 0 2 0 0 168.0 0 0 0 2 171.0 0 0 0 1 174.0 0 1 0 0 175.0 0 0 0 1 176.0 0 1 0 0 177.0 0 1 0 1 178.0 0 1 0 0 183.0 0 1 0 1 184.0 0 1 0 0 185.0 0 0 0 1 187.0 0 0 0 1 192.0 0 1 0 0 194.0 0 1 0 0 196.0 0 0 0 1 200.0 0 1 0 0 201.0 0 0 0 1 212.0 0 0 0 1 218.0 0 1 0 0 219.0 0 1 0 0 222.0 0 0 0 1 229.0 0 1 0 0 234.0 0 0 0 1 243.0 0 0 0 1 244.0 0 1 0 0 253.0 0 1 0 0 256.0 0 0 0 1 262.0 0 0 0 1 263.0 0 1 0 0 264.0 0 0 0 1 266.0 0 1 0 0 267.0 0 0 0 1 275.0 0 1 0 0 312.0 0 1 0 0 317.0 0 0 0 1 324.0 0 1 0 0 373.0 0 0 0 1 389.0 0 1 0 0 409.0 0 0 0 1 419.0 0 1 0 0 446.0 0 0 0 1 456.0 0 0 0 1 472.0 0 1 0 0 474.0 0 1 0 0 477.0 0 0 0 1 497.0 0 0 0 1 504.0 0 1 0 0 519.0 0 1 0 0 554.0 0 0 0 1 585.0 0 1 0 0 590.0 0 0 0 1 594.0 0 0 0 1 631.0 0 1 0 0 640.0 0 1 0 0 763.0 0 0 0 1 780.0 0 0 0 1 816.0 0 1 0 0 840.0 0 1 0 0