## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[P03-female_bleached_44.sorted.bam] OUTPUT=P03-female_bleached_44.sorted.dedup.bam METRICS_FILE=P03-female_bleached_44-picard_metrics.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Jul 18 12:07:42 PDT 2019 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 993262 21447569 0 2161572 528907 5837818 932064 0.278081 35744624 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.0332 11855052 0 12266898 2.0 1.60022 2581155 700870 2423313 3.0 1.9114 740196 83929 616547 4.0 2.082175 250999 14674 184798 5.0 2.175896 95540 3152 62754 6.0 2.227331 40070 782 24314 7.0 2.255558 18613 232 11063 8.0 2.271049 9467 76 5758 9.0 2.27955 5297 37 3449 10.0 2.284216 3156 15 2258 11.0 2.286776 2086 8 1571 12.0 2.288182 1483 3 1138 13.0 2.288953 1038 3 880 14.0 2.289376 794 3 715 15.0 2.289608 686 1 577 16.0 2.289736 502 2 427 17.0 2.289806 408 0 358 18.0 2.289844 348 0 337 19.0 2.289865 280 0 266 20.0 2.289877 273 1 250 21.0 2.289883 240 1 181 22.0 2.289886 190 1 162 23.0 2.289888 164 1 151 24.0 2.289889 127 1 127 25.0 2.28989 132 2 105 26.0 2.28989 101 0 99 27.0 2.289891 90 0 83 28.0 2.289891 86 1 79 29.0 2.289891 64 0 52 30.0 2.289891 60 1 67 31.0 2.289891 65 0 58 32.0 2.289891 52 0 42 33.0 2.289891 43 0 49 34.0 2.289891 45 0 41 35.0 2.289891 54 0 45 36.0 2.289891 44 0 40 37.0 2.289891 33 0 40 38.0 2.289891 39 0 34 39.0 2.289891 28 0 27 40.0 2.289891 25 0 30 41.0 2.289891 27 0 21 42.0 2.289891 38 0 28 43.0 2.289891 19 0 31 44.0 2.289891 29 0 19 45.0 2.289891 26 0 22 46.0 2.289891 23 0 19 47.0 2.289891 17 0 14 48.0 2.289891 13 0 14 49.0 2.289891 19 0 21 50.0 2.289891 17 0 10 51.0 2.289891 17 0 18 52.0 2.289891 15 0 14 53.0 2.289891 17 0 8 54.0 2.289891 9 0 8 55.0 2.289891 11 0 20 56.0 2.289891 15 0 10 57.0 2.289891 10 0 13 58.0 2.289891 9 0 9 59.0 2.289891 16 0 10 60.0 2.289891 4 0 7 61.0 2.289891 8 0 6 62.0 2.289891 8 0 2 63.0 2.289891 9 0 8 64.0 2.289891 3 0 3 65.0 2.289891 5 0 3 66.0 2.289891 3 0 4 67.0 2.289891 9 0 4 68.0 2.289891 2 0 5 69.0 2.289891 7 0 8 70.0 2.289891 1 0 5 71.0 2.289891 3 0 9 72.0 2.289891 10 0 6 73.0 2.289891 5 0 4 74.0 2.289891 11 0 7 75.0 2.289891 4 0 3 76.0 2.289891 4 0 2 77.0 2.289891 4 0 4 78.0 2.289891 5 0 4 79.0 2.289891 2 0 3 80.0 2.289891 0 0 4 81.0 2.289891 3 0 1 82.0 2.289891 3 0 3 83.0 2.289891 5 0 4 84.0 2.289891 4 0 4 85.0 2.289891 6 0 9 86.0 2.289891 3 0 6 87.0 2.289891 8 0 5 88.0 2.289891 8 0 3 89.0 2.289891 3 0 2 90.0 2.289891 3 0 0 91.0 2.289891 2 0 2 92.0 2.289891 2 0 2 93.0 2.289891 2 0 2 94.0 2.289891 1 0 4 95.0 2.289891 2 0 1 96.0 2.289891 1 0 6 97.0 2.289891 3 0 1 98.0 2.289891 5 0 2 99.0 2.289891 1 0 1 100.0 2.289891 0 0 1 101.0 0 3 0 1 102.0 0 0 0 2 103.0 0 3 0 2 104.0 0 1 0 0 105.0 0 3 0 1 106.0 0 0 0 2 107.0 0 1 0 3 108.0 0 2 0 3 109.0 0 3 0 1 110.0 0 2 0 2 111.0 0 2 0 4 112.0 0 0 0 1 113.0 0 2 0 3 114.0 0 4 0 0 115.0 0 4 0 1 116.0 0 0 0 1 117.0 0 3 0 2 118.0 0 1 0 2 119.0 0 0 0 1 120.0 0 3 0 0 122.0 0 1 0 3 123.0 0 1 0 3 124.0 0 1 0 1 125.0 0 2 0 1 126.0 0 1 0 1 127.0 0 2 0 0 128.0 0 0 0 1 129.0 0 2 0 0 130.0 0 1 0 1 131.0 0 0 0 1 133.0 0 1 0 1 134.0 0 0 0 1 135.0 0 1 0 1 136.0 0 0 0 1 137.0 0 1 0 2 138.0 0 0 0 1 139.0 0 0 0 2 140.0 0 2 0 0 141.0 0 1 0 1 142.0 0 1 0 1 143.0 0 2 0 1 144.0 0 2 0 0 145.0 0 3 0 2 146.0 0 1 0 1 147.0 0 1 0 1 148.0 0 0 0 3 149.0 0 1 0 0 150.0 0 0 0 2 151.0 0 2 0 1 152.0 0 1 0 0 153.0 0 1 0 2 155.0 0 3 0 0 157.0 0 0 0 1 158.0 0 3 0 0 159.0 0 1 0 0 160.0 0 0 0 1 163.0 0 0 0 1 166.0 0 1 0 0 168.0 0 1 0 0 169.0 0 0 0 1 170.0 0 1 0 0 174.0 0 0 0 1 177.0 0 0 0 1 181.0 0 1 0 0 185.0 0 1 0 0 186.0 0 0 0 1 187.0 0 1 0 0 189.0 0 1 0 0 193.0 0 0 0 1 196.0 0 0 0 1 199.0 0 1 0 0 205.0 0 1 0 0 207.0 0 0 0 1 212.0 0 1 0 1 215.0 0 0 0 1 216.0 0 0 0 1 219.0 0 1 0 0 221.0 0 1 0 0 222.0 0 0 0 1 223.0 0 2 0 0 232.0 0 0 0 1 235.0 0 0 0 1 236.0 0 1 0 0 245.0 0 0 0 1 250.0 0 1 0 0 258.0 0 1 0 0 259.0 0 0 0 1 266.0 0 1 0 0 280.0 0 0 0 1 285.0 0 0 0 1 287.0 0 0 0 1 294.0 0 1 0 0 295.0 0 1 0 0 306.0 0 1 0 0 317.0 0 0 0 1 319.0 0 0 0 1 327.0 0 1 0 0 328.0 0 1 0 0 352.0 0 0 0 1 361.0 0 1 0 0 382.0 0 0 0 1 403.0 0 1 0 0 415.0 0 0 0 1 439.0 0 1 0 0 467.0 0 0 0 1 481.0 0 1 0 0 513.0 0 0 0 1 542.0 0 1 0 0 559.0 0 0 0 1 579.0 0 1 0 0 687.0 0 0 0 1 714.0 0 1 0 0 906.0 0 0 0 1 930.0 0 1 0 0