## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[P01-male_bleached_K5.sorted.bam] OUTPUT=P01-male_bleached_K5.sorted.dedup.bam METRICS_FILE=P01-male_bleached_K5-picard_metrics.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Jul 18 10:52:26 PDT 2019 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 944593 23233066 0 2283111 631301 9613021 761317 0.418837 20400182 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.018235 8553720 0 8740327 2.0 1.344256 2896214 569018 2890215 3.0 1.448642 1147118 68781 1101158 4.0 1.482064 503846 12561 460179 5.0 1.492766 238913 2828 207603 6.0 1.496192 120166 703 98115 7.0 1.497289 63343 216 49321 8.0 1.49764 34913 63 26026 9.0 1.497753 20070 34 14384 10.0 1.497789 12069 13 8795 11.0 1.4978 7544 4 5498 12.0 1.497804 4893 1 3727 13.0 1.497805 3366 2 2679 14.0 1.497806 2508 2 2037 15.0 1.497806 1842 0 1486 16.0 1.497806 1413 0 1173 17.0 1.497806 1114 0 947 18.0 1.497806 907 0 856 19.0 1.497806 752 0 682 20.0 1.497806 618 0 565 21.0 1.497806 547 0 460 22.0 1.497806 450 0 431 23.0 1.497806 399 0 339 24.0 1.497806 338 0 327 25.0 1.497806 294 0 252 26.0 1.497806 248 0 208 27.0 1.497806 195 0 183 28.0 1.497806 201 0 179 29.0 1.497806 162 0 182 30.0 1.497806 148 0 136 31.0 1.497806 143 0 124 32.0 1.497806 144 0 125 33.0 1.497806 105 0 96 34.0 1.497806 94 0 84 35.0 1.497806 77 0 62 36.0 1.497806 77 0 71 37.0 1.497806 63 0 67 38.0 1.497806 83 0 66 39.0 1.497806 66 0 65 40.0 1.497806 65 0 47 41.0 1.497806 45 0 42 42.0 1.497806 41 0 42 43.0 1.497806 35 0 33 44.0 1.497806 39 0 44 45.0 1.497806 28 0 30 46.0 1.497806 36 0 36 47.0 1.497806 36 0 27 48.0 1.497806 30 0 19 49.0 1.497806 24 0 21 50.0 1.497806 20 0 20 51.0 1.497806 24 0 22 52.0 1.497806 16 0 24 53.0 1.497806 20 0 21 54.0 1.497806 21 0 20 55.0 1.497806 17 0 22 56.0 1.497806 14 0 9 57.0 1.497806 17 0 13 58.0 1.497806 18 0 18 59.0 1.497806 19 0 12 60.0 1.497806 16 0 11 61.0 1.497806 10 0 10 62.0 1.497806 11 0 14 63.0 1.497806 11 0 10 64.0 1.497806 15 0 13 65.0 1.497806 9 0 10 66.0 1.497806 6 0 13 67.0 1.497806 14 0 7 68.0 1.497806 14 0 17 69.0 1.497806 13 0 13 70.0 1.497806 8 0 9 71.0 1.497806 17 0 8 72.0 1.497806 5 0 5 73.0 1.497806 7 0 2 74.0 1.497806 3 0 6 75.0 1.497806 5 0 1 76.0 1.497806 2 0 2 77.0 1.497806 6 0 4 78.0 1.497806 3 0 9 79.0 1.497806 4 0 2 80.0 1.497806 5 0 2 81.0 1.497806 6 0 3 82.0 1.497806 6 0 4 83.0 1.497806 0 0 2 84.0 1.497806 1 0 5 85.0 1.497806 5 0 7 86.0 1.497806 2 0 3 87.0 1.497806 5 0 3 88.0 1.497806 5 0 3 89.0 1.497806 4 0 3 90.0 1.497806 5 0 1 91.0 1.497806 2 0 1 92.0 1.497806 3 0 3 93.0 1.497806 1 0 4 94.0 1.497806 4 0 5 95.0 1.497806 2 0 1 96.0 1.497806 2 0 1 97.0 1.497806 4 0 1 98.0 1.497806 0 0 1 99.0 1.497806 0 0 1 100.0 1.497806 1 0 0 101.0 0 0 0 1 102.0 0 1 0 1 103.0 0 1 0 3 104.0 0 0 0 1 105.0 0 2 0 0 106.0 0 1 0 2 107.0 0 0 0 1 108.0 0 3 0 4 109.0 0 3 0 1 110.0 0 1 0 2 111.0 0 2 0 1 112.0 0 2 0 1 113.0 0 1 0 2 114.0 0 3 0 0 116.0 0 1 0 0 117.0 0 1 0 1 118.0 0 0 0 2 120.0 0 3 0 2 121.0 0 1 0 0 124.0 0 0 0 1 125.0 0 1 0 0 126.0 0 1 0 0 127.0 0 0 0 4 130.0 0 2 0 2 131.0 0 1 0 1 132.0 0 2 0 0 133.0 0 1 0 1 139.0 0 0 0 1 140.0 0 1 0 1 141.0 0 0 0 3 142.0 0 1 0 1 143.0 0 2 0 1 144.0 0 1 0 1 146.0 0 1 0 0 147.0 0 2 0 0 148.0 0 2 0 2 150.0 0 1 0 0 151.0 0 1 0 1 154.0 0 1 0 1 157.0 0 1 0 2 158.0 0 0 0 1 160.0 0 1 0 0 161.0 0 0 0 1 162.0 0 2 0 0 163.0 0 0 0 1 164.0 0 1 0 0 169.0 0 0 0 1 170.0 0 1 0 2 174.0 0 0 0 1 175.0 0 0 0 1 176.0 0 1 0 0 177.0 0 1 0 0 178.0 0 1 0 0 180.0 0 1 0 0 181.0 0 1 0 0 184.0 0 0 0 1 185.0 0 0 0 1 186.0 0 0 0 1 190.0 0 0 0 1 191.0 0 2 0 0 192.0 0 1 0 0 193.0 0 1 0 0 195.0 0 0 0 1 204.0 0 1 0 0 205.0 0 0 0 1 208.0 0 1 0 0 210.0 0 0 0 1 213.0 0 0 0 1 216.0 0 1 0 0 217.0 0 1 0 0 222.0 0 1 0 2 226.0 0 1 0 0 233.0 0 0 0 1 239.0 0 1 0 0 246.0 0 0 0 1 248.0 0 1 0 0 258.0 0 0 0 1 267.0 0 1 0 0 286.0 0 0 0 1 293.0 0 1 0 0 300.0 0 0 0 1 301.0 0 1 0 0 333.0 0 1 0 1 370.0 0 1 0 1