CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P08-CTTGTA-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P08-CTTGTA-READ2-Sequences.txt.gz_val_2.fq.gz) -o b_F_K5_04 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { b_F_K5_04 } ### [ threads ] => { 23 } Logs will be written to b_F_K5_04/logs [2019-03-14 21:12:47.387] [jointLog] [info] parsing read library format [2019-03-14 21:12:47.387] [jointLog] [info] There is 1 library. [2019-03-14 21:12:47.450] [stderrLog] [info] Loading Suffix Array [2019-03-14 21:12:47.450] [jointLog] [info] Loading Quasi index [2019-03-14 21:12:47.450] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 21:12:47.483] [stderrLog] [info] Loading Transcript Info [2019-03-14 21:12:47.492] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 21:12:47.495] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 21:12:47.497] [stderrLog] [info] Computing transcript lengths [2019-03-14 21:12:47.497] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 21:12:47.835] [jointLog] [info] done [2019-03-14 21:12:47.835] [jointLog] [info] Index contained 5838 targets [2019-03-14 21:12:47.835] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 96405, hits per frag: 0.229877 processed 1000002 fragments hits: 192608, hits per frag: 0.195235 processed 1500000 fragments hits: 289426, hits per frag: 0.197389 processed 2000001 fragments hits: 385688, hits per frag: 0.196246 processed 2500000 fragments hits: 481916, hits per frag: 0.195897 processed 3000000 fragments hits: 577832, hits per frag: 0.193303 processed 3500000 fragments hits: 673436, hits per frag: 0.195744 processed 4000000 fragments hits: 770521, hits per frag: 0.197806 processed 4500000 fragments hits: 867650, hits per frag: 0.196804 processed 5000000 fragments hits: 963783, hits per frag: 0.194861 processed 5500000 fragments hits: 1060885, hits per frag: 0.196025 processed 6000000 fragments hits: 1157680, hits per frag: 0.195531 processed 6500000 fragments hits: 1253645, hits per frag: 0.193796 processed 7000000 fragments hits: 1351193, hits per frag: 0.194221 processed 7500000 fragments hits: 1447913, hits per frag: 0.193499 processed 8000000 fragments hits: 1543269, hits per frag: 0.19342 processed 8500000 fragments hits: 1638938, hits per frag: 0.195079 processed 9000000 fragments hits: 1735078, hits per frag: 0.194848 processed 9500000 fragments hits: 1831512, hits per frag: 0.194674 processed 10000001 fragments hits: 1926836, hits per frag: 0.193175 processed 10500000 fragments hits: 2022014, hits per frag: 0.193089 processed 11000000 fragments hits: 2118411, hits per frag: 0.192984 processed 11500000 fragments hits: 2215205, hits per frag: 0.194407 processed 12000000 fragments hits: 2311953, hits per frag: 0.193106 processed 12500001 fragments hits: 2406342, hits per frag: 0.192849 processed 13000000 fragments hits: 2501303, hits per frag: 0.194106 processed 13500001 fragments hits: 2597628, hits per frag: 0.192524 processed 14000000 fragments hits: 2694107, hits per frag: 0.19277 processed 14500000 fragments hits: 2790495, hits per frag: 0.193809 processed 15000000 fragments hits: 2887056, hits per frag: 0.193551 processed 15500000 fragments hits: 2982998, hits per frag: 0.193164 processed 16000001 fragments hits: 3078675, hits per frag: 0.193104 processed 16500000 fragments hits: 3174668, hits per frag: 0.193125 processed 17000001 fragments hits: 3270783, hits per frag: 0.192786 processed 17500001 fragments hits: 3366899, hits per frag: 0.193288 processed 18000001 fragments hits: 3463015, hits per frag: 0.192968 processed 18500000 fragments hits: 3558799, hits per frag: 0.192867 processed 19000000 fragments hits: 3655523, hits per frag: 0.192714 processed 19500000 fragments hits: 3751212, hits per frag: 0.193043 processed 20000000 fragments hits: 3847928, hits per frag: 0.193333 processed 20500000 fragments hits: 3944476, hits per frag: 0.192855 processed 21000000 fragments hits: 4040784, hits per frag: 0.193528 processed 21500001 fragments hits: 4137724, hits per frag: 0.19299 processed 22000000 fragments hits: 4233659, hits per frag: 0.19356 processed 22500001 fragments hits: 4329702, hits per frag: 0.193265 processed 23000000 fragments hits: 4426415, hits per frag: 0.193025 processed 23500000 fragments hits: 4522474, hits per frag: 0.192885 processed 24000000 fragments hits: 4618628, hits per frag: 0.193091 processed 24500000 fragments hits: 4715068, hits per frag: 0.19269 processed 25000000 fragments hits: 4812227, hits per frag: 0.193138 processed 25500000 fragments hits: 4908470, hits per frag: 0.19322 processed 26000000 fragments hits: 5004211, hits per frag: 0.192568 processed 26500000 fragments hits: 5101321, hits per frag: 0.193189 processed 27000001 fragments hits: 5197073, hits per frag: 0.192753 processed 27500000 fragments hits: 5292224, hits per frag: 0.192992 processed 28000000 fragments hits: 5389062, hits per frag: 0.192914 [2019-03-14 21:13:55.812] [jointLog] [info] Computed 7401 rich equivalence classes for further processing [2019-03-14 21:13:55.812] [jointLog] [info] Counted 2716216 total reads in the equivalence classes [2019-03-14 21:13:55.823] [jointLog] [warning] 0.0118102% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 21:13:55.823] [jointLog] [warning] Only 2716216 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 21:13:55.823] [jointLog] [info] Mapping rate = 9.59013% [2019-03-14 21:13:55.824] [jointLog] [info] finished quantifyLibrary() [2019-03-14 21:13:55.824] [jointLog] [info] Starting optimizer [2019-03-14 21:13:55.829] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 21:13:55.829] [jointLog] [info] iteration = 0 | max rel diff. = 623.186 [2019-03-14 21:13:55.848] [jointLog] [info] iteration = 100 | max rel diff. = 0.126921 [2019-03-14 21:13:55.866] [jointLog] [info] iteration = 200 | max rel diff. = 0.0631581 [2019-03-14 21:13:55.885] [jointLog] [info] iteration = 300 | max rel diff. = 0.0433758 [2019-03-14 21:13:55.903] [jointLog] [info] iteration = 400 | max rel diff. = 0.0310137 [2019-03-14 21:13:55.920] [jointLog] [info] iteration = 500 | max rel diff. = 0.0246268 [2019-03-14 21:13:55.938] [jointLog] [info] iteration = 600 | max rel diff. = 0.0179093 [2019-03-14 21:13:55.956] [jointLog] [info] iteration = 700 | max rel diff. = 0.0152061 [2019-03-14 21:13:55.974] [jointLog] [info] iteration = 800 | max rel diff. = 0.0120247 [2019-03-14 21:13:55.991] [jointLog] [info] iteration = 900 | max rel diff. = 0.0120261 [2019-03-14 21:13:56.007] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0110934 [2019-03-14 21:13:56.012] [jointLog] [info] iteration = 1034 | max rel diff. = 0.00984526 [2019-03-14 21:13:56.012] [jointLog] [info] Finished optimizer [2019-03-14 21:13:56.012] [jointLog] [info] writing output [2019-03-14 21:13:56.023] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: b_F_K5_04/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl b_F_K5_04/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > b_F_K5_04/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P09-GTGAAA-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P09-GTGAAA-READ2-Sequences.txt.gz_val_2.fq.gz) -o b_F_K5_05 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { b_F_K5_05 } ### [ threads ] => { 23 } Logs will be written to b_F_K5_05/logs [2019-03-14 21:13:56.496] [jointLog] [info] parsing read library format [2019-03-14 21:13:56.496] [jointLog] [info] There is 1 library. [2019-03-14 21:13:56.558] [stderrLog] [info] Loading Suffix Array [2019-03-14 21:13:56.558] [jointLog] [info] Loading Quasi index [2019-03-14 21:13:56.558] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 21:13:56.597] [stderrLog] [info] Loading Transcript Info [2019-03-14 21:13:56.606] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 21:13:56.609] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 21:13:56.611] [stderrLog] [info] Computing transcript lengths [2019-03-14 21:13:56.611] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 21:13:56.948] [jointLog] [info] done [2019-03-14 21:13:56.948] [jointLog] [info] Index contained 5838 targets [2019-03-14 21:13:56.948] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 88704, hits per frag: 0.208844 processed 1000000 fragments hits: 177946, hits per frag: 0.193161 processed 1500000 fragments hits: 265438, hits per frag: 0.180362 processed 2000001 fragments hits: 354078, hits per frag: 0.1839 processed 2500000 fragments hits: 442349, hits per frag: 0.181893 processed 3000000 fragments hits: 531422, hits per frag: 0.181326 processed 3500001 fragments hits: 619397, hits per frag: 0.182259 processed 4000000 fragments hits: 706963, hits per frag: 0.179619 processed 4500000 fragments hits: 796393, hits per frag: 0.178929 processed 5000000 fragments hits: 884745, hits per frag: 0.177856 processed 5500001 fragments hits: 972845, hits per frag: 0.180156 processed 6000000 fragments hits: 1061253, hits per frag: 0.179793 processed 6500000 fragments hits: 1150043, hits per frag: 0.180114 processed 7000000 fragments hits: 1238273, hits per frag: 0.178942 processed 7500000 fragments hits: 1326021, hits per frag: 0.178635 processed 8000000 fragments hits: 1414033, hits per frag: 0.178323 processed 8500000 fragments hits: 1502639, hits per frag: 0.17864 processed 9000001 fragments hits: 1591887, hits per frag: 0.177862 processed 9500000 fragments hits: 1680415, hits per frag: 0.179963 processed 10000000 fragments hits: 1768823, hits per frag: 0.178002 processed 10500000 fragments hits: 1856617, hits per frag: 0.178445 processed 11000000 fragments hits: 1943664, hits per frag: 0.177135 processed 11500000 fragments hits: 2031771, hits per frag: 0.178091 processed 12000000 fragments hits: 2119293, hits per frag: 0.17759 processed 12500000 fragments hits: 2207320, hits per frag: 0.178563 processed 13000000 fragments hits: 2294847, hits per frag: 0.177731 processed 13500000 fragments hits: 2382828, hits per frag: 0.177176 processed 14000000 fragments hits: 2470901, hits per frag: 0.177308 processed 14500000 fragments hits: 2559429, hits per frag: 0.177238 processed 15000000 fragments hits: 2647939, hits per frag: 0.17703 processed 15500000 fragments hits: 2735964, hits per frag: 0.176892 processed 16000001 fragments hits: 2824312, hits per frag: 0.177413 processed 16500000 fragments hits: 2913140, hits per frag: 0.176749 processed 17000001 fragments hits: 3001400, hits per frag: 0.17724 processed 17500000 fragments hits: 3089615, hits per frag: 0.176742 processed 18000001 fragments hits: 3177730, hits per frag: 0.176803 processed 18500000 fragments hits: 3264990, hits per frag: 0.176981 processed 19000000 fragments hits: 3352323, hits per frag: 0.177243 processed 19500000 fragments hits: 3440169, hits per frag: 0.177071 processed 20000000 fragments hits: 3527616, hits per frag: 0.177222 processed 20500000 fragments hits: 3615216, hits per frag: 0.177107 processed 21000000 fragments hits: 3702956, hits per frag: 0.176564 processed 21500000 fragments hits: 3791464, hits per frag: 0.177156 [2019-03-14 21:14:49.124] [jointLog] [info] Computed 6922 rich equivalence classes for further processing [2019-03-14 21:14:49.124] [jointLog] [info] Counted 1916701 total reads in the equivalence classes [2019-03-14 21:14:49.135] [jointLog] [warning] 0.0194906% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 21:14:49.135] [jointLog] [warning] Only 1916701 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 21:14:49.135] [jointLog] [info] Mapping rate = 8.75913% [2019-03-14 21:14:49.135] [jointLog] [info] finished quantifyLibrary() [2019-03-14 21:14:49.136] [jointLog] [info] Starting optimizer [2019-03-14 21:14:49.140] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 21:14:49.140] [jointLog] [info] iteration = 0 | max rel diff. = 428.646 [2019-03-14 21:14:49.156] [jointLog] [info] iteration = 100 | max rel diff. = 0.112607 [2019-03-14 21:14:49.171] [jointLog] [info] iteration = 200 | max rel diff. = 0.0561126 [2019-03-14 21:14:49.186] [jointLog] [info] iteration = 300 | max rel diff. = 0.0366474 [2019-03-14 21:14:49.201] [jointLog] [info] iteration = 400 | max rel diff. = 0.0243107 [2019-03-14 21:14:49.216] [jointLog] [info] iteration = 500 | max rel diff. = 0.0243121 [2019-03-14 21:14:49.233] [jointLog] [info] iteration = 600 | max rel diff. = 0.0175144 [2019-03-14 21:14:49.249] [jointLog] [info] iteration = 700 | max rel diff. = 0.0150948 [2019-03-14 21:14:49.266] [jointLog] [info] iteration = 800 | max rel diff. = 0.0129998 [2019-03-14 21:14:49.283] [jointLog] [info] iteration = 900 | max rel diff. = 0.0114931 [2019-03-14 21:14:49.299] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0114934 [2019-03-14 21:14:49.311] [jointLog] [info] iteration = 1072 | max rel diff. = 0.00919436 [2019-03-14 21:14:49.312] [jointLog] [info] Finished optimizer [2019-03-14 21:14:49.312] [jointLog] [info] writing output [2019-03-14 21:14:49.323] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: b_F_K5_05/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl b_F_K5_05/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > b_F_K5_05/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P13-ATCACG-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P13-ATCACG-READ2-Sequences.txt.gz_val_2.fq.gz) -o b_F_K5_06 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { b_F_K5_06 } ### [ threads ] => { 23 } Logs will be written to b_F_K5_06/logs [2019-03-14 21:14:49.808] [jointLog] [info] parsing read library format [2019-03-14 21:14:49.808] [jointLog] [info] There is 1 library. [2019-03-14 21:14:49.871] [stderrLog] [info] Loading Suffix Array [2019-03-14 21:14:49.871] [jointLog] [info] Loading Quasi index [2019-03-14 21:14:49.871] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 21:14:49.908] [stderrLog] [info] Loading Transcript Info [2019-03-14 21:14:49.917] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 21:14:49.920] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 21:14:49.922] [stderrLog] [info] Computing transcript lengths [2019-03-14 21:14:49.922] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 21:14:50.262] [jointLog] [info] done [2019-03-14 21:14:50.262] [jointLog] [info] Index contained 5838 targets [2019-03-14 21:14:50.262] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 74570, hits per frag: 0.182204 processed 1000000 fragments hits: 149554, hits per frag: 0.153446 processed 1500000 fragments hits: 224725, hits per frag: 0.152215 processed 2000000 fragments hits: 298991, hits per frag: 0.154634 processed 2500000 fragments hits: 373667, hits per frag: 0.150859 processed 3000000 fragments hits: 447898, hits per frag: 0.151659 processed 3500000 fragments hits: 522364, hits per frag: 0.152265 processed 4000001 fragments hits: 595364, hits per frag: 0.150692 processed 4500001 fragments hits: 669413, hits per frag: 0.152919 processed 5000000 fragments hits: 744479, hits per frag: 0.151729 processed 5500000 fragments hits: 819804, hits per frag: 0.150397 processed 6000000 fragments hits: 894796, hits per frag: 0.150933 processed 6500000 fragments hits: 968530, hits per frag: 0.149036 processed 7000001 fragments hits: 1042073, hits per frag: 0.150168 processed 7500001 fragments hits: 1116308, hits per frag: 0.150234 processed 8000000 fragments hits: 1189798, hits per frag: 0.14982 processed 8500000 fragments hits: 1262832, hits per frag: 0.148961 processed 9000000 fragments hits: 1338113, hits per frag: 0.14917 processed 9500000 fragments hits: 1413026, hits per frag: 0.14944 processed 10000000 fragments hits: 1487446, hits per frag: 0.150053 processed 10500000 fragments hits: 1561615, hits per frag: 0.149531 processed 11000000 fragments hits: 1635769, hits per frag: 0.148776 processed 11500000 fragments hits: 1710084, hits per frag: 0.149214 processed 12000001 fragments hits: 1783769, hits per frag: 0.148704 processed 12500000 fragments hits: 1857527, hits per frag: 0.149312 processed 13000000 fragments hits: 1930681, hits per frag: 0.149485 processed 13500002 fragments hits: 2006531, hits per frag: 0.149411 processed 14000000 fragments hits: 2081173, hits per frag: 0.148991 processed 14500000 fragments hits: 2156316, hits per frag: 0.149265 processed 15000000 fragments hits: 2230437, hits per frag: 0.149605 processed 15500000 fragments hits: 2304392, hits per frag: 0.149759 processed 16000000 fragments hits: 2377829, hits per frag: 0.149315 processed 16500000 fragments hits: 2451296, hits per frag: 0.149157 processed 17000001 fragments hits: 2524287, hits per frag: 0.149071 [2019-03-14 21:15:29.263] [jointLog] [info] Computed 6627 rich equivalence classes for further processing [2019-03-14 21:15:29.263] [jointLog] [info] Counted 1294477 total reads in the equivalence classes [2019-03-14 21:15:29.273] [jointLog] [warning] 0.0100547% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 21:15:29.273] [jointLog] [warning] Only 1294477 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 21:15:29.273] [jointLog] [info] Mapping rate = 7.48881% [2019-03-14 21:15:29.273] [jointLog] [info] finished quantifyLibrary() [2019-03-14 21:15:29.273] [jointLog] [info] Starting optimizer [2019-03-14 21:15:29.279] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 21:15:29.279] [jointLog] [info] iteration = 0 | max rel diff. = 305.923 [2019-03-14 21:15:29.295] [jointLog] [info] iteration = 100 | max rel diff. = 0.113946 [2019-03-14 21:15:29.311] [jointLog] [info] iteration = 200 | max rel diff. = 0.0606231 [2019-03-14 21:15:29.326] [jointLog] [info] iteration = 300 | max rel diff. = 0.0432692 [2019-03-14 21:15:29.340] [jointLog] [info] iteration = 400 | max rel diff. = 0.0293289 [2019-03-14 21:15:29.354] [jointLog] [info] iteration = 500 | max rel diff. = 0.0218005 [2019-03-14 21:15:29.368] [jointLog] [info] iteration = 600 | max rel diff. = 0.017429 [2019-03-14 21:15:29.383] [jointLog] [info] iteration = 700 | max rel diff. = 0.0147314 [2019-03-14 21:15:29.397] [jointLog] [info] iteration = 800 | max rel diff. = 0.0118523 [2019-03-14 21:15:29.413] [jointLog] [info] iteration = 900 | max rel diff. = 0.0111513 [2019-03-14 21:15:29.429] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0113568 [2019-03-14 21:15:29.445] [jointLog] [info] iteration = 1091 | max rel diff. = 0.00876297 [2019-03-14 21:15:29.445] [jointLog] [info] Finished optimizer [2019-03-14 21:15:29.445] [jointLog] [info] writing output [2019-03-14 21:15:29.456] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: b_F_K5_06/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl b_F_K5_06/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > b_F_K5_06/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P02-ACAGTG-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P02-ACAGTG-READ2-Sequences.txt.gz_val_2.fq.gz) -o nb_F_K5_07 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { nb_F_K5_07 } ### [ threads ] => { 23 } Logs will be written to nb_F_K5_07/logs [2019-03-14 21:15:29.701] [jointLog] [info] parsing read library format [2019-03-14 21:15:29.701] [jointLog] [info] There is 1 library. [2019-03-14 21:15:29.762] [stderrLog] [info] Loading Suffix Array [2019-03-14 21:15:29.762] [jointLog] [info] Loading Quasi index [2019-03-14 21:15:29.762] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 21:15:29.802] [stderrLog] [info] Loading Transcript Info [2019-03-14 21:15:29.812] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 21:15:29.816] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 21:15:29.817] [stderrLog] [info] Computing transcript lengths [2019-03-14 21:15:29.817] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 21:15:30.153] [jointLog] [info] done [2019-03-14 21:15:30.153] [jointLog] [info] Index contained 5838 targets [2019-03-14 21:15:30.153] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 98567, hits per frag: 0.216033 processed 1000000 fragments hits: 198060, hits per frag: 0.219587 processed 1500000 fragments hits: 295917, hits per frag: 0.205702 processed 2000001 fragments hits: 396436, hits per frag: 0.198904 processed 2500001 fragments hits: 495814, hits per frag: 0.204032 processed 3000001 fragments hits: 595391, hits per frag: 0.206629 processed 3500000 fragments hits: 694628, hits per frag: 0.200206 processed 4000000 fragments hits: 793730, hits per frag: 0.202967 processed 4500000 fragments hits: 891919, hits per frag: 0.202764 processed 5000000 fragments hits: 990814, hits per frag: 0.201485 processed 5500002 fragments hits: 1089787, hits per frag: 0.202345 processed 6000000 fragments hits: 1189232, hits per frag: 0.201372 processed 6500000 fragments hits: 1288314, hits per frag: 0.200353 processed 7000000 fragments hits: 1386980, hits per frag: 0.19892 processed 7500000 fragments hits: 1485377, hits per frag: 0.198842 processed 8000000 fragments hits: 1584438, hits per frag: 0.199471 processed 8500000 fragments hits: 1683802, hits per frag: 0.200062 processed 9000000 fragments hits: 1782859, hits per frag: 0.199914 processed 9500001 fragments hits: 1881122, hits per frag: 0.199543 processed 10000003 fragments hits: 1981100, hits per frag: 0.198779 processed 10500001 fragments hits: 2079039, hits per frag: 0.199729 processed 11500001 fragments hits: 2276828, hits per frag: 0.199153 processed 12000000 fragments hits: 2375320, hits per frag: 0.199425 processed 12500001 fragments hits: 2473825, hits per frag: 0.199708 processed 13000000 fragments hits: 2572711, hits per frag: 0.198831 processed 13500000 fragments hits: 2670226, hits per frag: 0.199229 processed 14000000 fragments hits: 2768420, hits per frag: 0.19864 processed 14500000 fragments hits: 2866561, hits per frag: 0.198543 processed 15000000 fragments hits: 2965753, hits per frag: 0.198092 processed 15500000 fragments hits: 3065192, hits per frag: 0.19868 processed 16000000 fragments hits: 3164345, hits per frag: 0.198338 processed 16500000 fragments hits: 3262478, hits per frag: 0.198506 processed 17000000 fragments hits: 3360918, hits per frag: 0.1988 processed 17500000 fragments hits: 3461286, hits per frag: 0.198785 processed 18000000 fragments hits: 3560643, hits per frag: 0.198105 processed 18500000 fragments hits: 3658846, hits per frag: 0.198515 processed 19000000 fragments hits: 3758151, hits per frag: 0.199283 processed 19500000 fragments hits: 3857701, hits per frag: 0.19836 processed 20000001 fragments hits: 3956222, hits per frag: 0.197992 processed 20500000 fragments hits: 4055280, hits per frag: 0.198316 processed 21000001 fragments hits: 4154031, hits per frag: 0.198401 processed 21500000 fragments hits: 4253258, hits per frag: 0.198526 processed 22000000 fragments hits: 4352877, hits per frag: 0.198505 processed 22500000 fragments hits: 4452307, hits per frag: 0.198633 processed 23000000 fragments hits: 4551502, hits per frag: 0.198745 processed 23500000 fragments hits: 4649877, hits per frag: 0.198362 [2019-03-14 21:16:25.358] [jointLog] [info] Computed 7091 rich equivalence classes for further processing [2019-03-14 21:16:25.358] [jointLog] [info] Counted 2346516 total reads in the equivalence classes [2019-03-14 21:16:25.369] [jointLog] [warning] 0.0207925% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 21:16:25.369] [jointLog] [warning] Only 2346516 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 21:16:25.369] [jointLog] [info] Mapping rate = 9.83271% [2019-03-14 21:16:25.369] [jointLog] [info] finished quantifyLibrary() [2019-03-14 21:16:25.370] [jointLog] [info] Starting optimizer [2019-03-14 21:16:25.375] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 21:16:25.376] [jointLog] [info] iteration = 0 | max rel diff. = 538.307 [2019-03-14 21:16:25.402] [jointLog] [info] iteration = 100 | max rel diff. = 0.118479 [2019-03-14 21:16:25.419] [jointLog] [info] iteration = 200 | max rel diff. = 0.0574428 [2019-03-14 21:16:25.437] [jointLog] [info] iteration = 300 | max rel diff. = 0.0391362 [2019-03-14 21:16:25.455] [jointLog] [info] iteration = 400 | max rel diff. = 0.0273949 [2019-03-14 21:16:25.473] [jointLog] [info] iteration = 500 | max rel diff. = 0.0220548 [2019-03-14 21:16:25.491] [jointLog] [info] iteration = 600 | max rel diff. = 0.019218 [2019-03-14 21:16:25.509] [jointLog] [info] iteration = 700 | max rel diff. = 0.0157131 [2019-03-14 21:16:25.526] [jointLog] [info] iteration = 800 | max rel diff. = 0.013467 [2019-03-14 21:16:25.544] [jointLog] [info] iteration = 900 | max rel diff. = 0.0111952 [2019-03-14 21:16:25.562] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0107554 [2019-03-14 21:16:25.574] [jointLog] [info] iteration = 1068 | max rel diff. = 0.00971556 [2019-03-14 21:16:25.575] [jointLog] [info] Finished optimizer [2019-03-14 21:16:25.575] [jointLog] [info] writing output [2019-03-14 21:16:25.586] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: nb_F_K5_07/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl nb_F_K5_07/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > nb_F_K5_07/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P10-TAGCTT-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P10-TAGCTT-READ2-Sequences.txt.gz_val_2.fq.gz) -o nb_F_K5_08 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { nb_F_K5_08 } ### [ threads ] => { 23 } Logs will be written to nb_F_K5_08/logs [2019-03-14 21:16:25.815] [jointLog] [info] parsing read library format [2019-03-14 21:16:25.815] [jointLog] [info] There is 1 library. [2019-03-14 21:16:25.876] [stderrLog] [info] Loading Suffix Array [2019-03-14 21:16:25.876] [jointLog] [info] Loading Quasi index [2019-03-14 21:16:25.876] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 21:16:25.910] [stderrLog] [info] Loading Transcript Info [2019-03-14 21:16:25.919] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 21:16:25.923] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 21:16:25.924] [stderrLog] [info] Computing transcript lengths [2019-03-14 21:16:25.924] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 21:16:26.263] [jointLog] [info] done [2019-03-14 21:16:26.263] [jointLog] [info] Index contained 5838 targets [2019-03-14 21:16:26.263] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 85063, hits per frag: 0.200587 processed 1000000 fragments hits: 169837, hits per frag: 0.186069 processed 1500000 fragments hits: 255150, hits per frag: 0.176947 processed 2000000 fragments hits: 339748, hits per frag: 0.173201 processed 2500001 fragments hits: 425559, hits per frag: 0.174427 processed 3000000 fragments hits: 510692, hits per frag: 0.170955 processed 3500001 fragments hits: 596800, hits per frag: 0.172096 processed 4000000 fragments hits: 681973, hits per frag: 0.171529 processed 4500000 fragments hits: 766839, hits per frag: 0.176655 processed 5000001 fragments hits: 851562, hits per frag: 0.173012 processed 5500001 fragments hits: 937385, hits per frag: 0.171679 processed 6000000 fragments hits: 1022817, hits per frag: 0.173508 processed 6500000 fragments hits: 1108132, hits per frag: 0.17186 processed 7000000 fragments hits: 1193928, hits per frag: 0.173325 processed 7500000 fragments hits: 1278935, hits per frag: 0.172519 processed 8000000 fragments hits: 1365184, hits per frag: 0.172334 processed 8500000 fragments hits: 1449790, hits per frag: 0.17127 processed 9000000 fragments hits: 1535530, hits per frag: 0.17372 processed 9500001 fragments hits: 1621186, hits per frag: 0.170865 processed 10000000 fragments hits: 1706659, hits per frag: 0.173302 processed 10500000 fragments hits: 1792435, hits per frag: 0.171722 processed 11000001 fragments hits: 1877497, hits per frag: 0.171665 processed 11500000 fragments hits: 1962495, hits per frag: 0.171955 processed 12500000 fragments hits: 2132545, hits per frag: 0.17112 processed 13000000 fragments hits: 2217753, hits per frag: 0.171715 processed 13500000 fragments hits: 2303039, hits per frag: 0.17142 processed 14000000 fragments hits: 2387390, hits per frag: 0.171416 processed 14500001 fragments hits: 2473292, hits per frag: 0.170967 processed 15000001 fragments hits: 2557499, hits per frag: 0.17153 processed 15500000 fragments hits: 2642234, hits per frag: 0.17083 processed 16000000 fragments hits: 2726493, hits per frag: 0.171545 processed 16500001 fragments hits: 2811778, hits per frag: 0.171132 processed 17000000 fragments hits: 2897568, hits per frag: 0.170675 processed 17500000 fragments hits: 2984186, hits per frag: 0.170879 processed 18000003 fragments hits: 3068271, hits per frag: 0.170574 processed 18500000 fragments hits: 3153065, hits per frag: 0.171505 processed 19000000 fragments hits: 3238300, hits per frag: 0.171192 processed 19500001 fragments hits: 3323154, hits per frag: 0.1713 processed 20000001 fragments hits: 3409452, hits per frag: 0.170774 processed 20500000 fragments hits: 3494749, hits per frag: 0.170861 processed 21000000 fragments hits: 3579223, hits per frag: 0.170889 processed 21500000 fragments hits: 3664780, hits per frag: 0.171061 processed 22000000 fragments hits: 3749762, hits per frag: 0.170813 processed 22500000 fragments hits: 3834838, hits per frag: 0.170784 processed 23000000 fragments hits: 3921099, hits per frag: 0.171105 processed 23500001 fragments hits: 4006721, hits per frag: 0.170803 processed 24000002 fragments hits: 4092591, hits per frag: 0.170778 processed 24500000 fragments hits: 4177843, hits per frag: 0.171417 processed 25000001 fragments hits: 4264408, hits per frag: 0.17119 processed 25500000 fragments hits: 4350663, hits per frag: 0.171135 processed 26000000 fragments hits: 4436347, hits per frag: 0.17075 processed 26500001 fragments hits: 4519575, hits per frag: 0.171264 processed 27000000 fragments hits: 4605079, hits per frag: 0.170938 processed 27500000 fragments hits: 4689385, hits per frag: 0.171484 processed 28000000 fragments hits: 4775319, hits per frag: 0.170607 processed 28500000 fragments hits: 4859846, hits per frag: 0.170984 processed 29000000 fragments hits: 4945177, hits per frag: 0.170964 processed 29500000 fragments hits: 5031851, hits per frag: 0.170702 processed 30000000 fragments hits: 5116702, hits per frag: 0.171376 processed 30500000 fragments hits: 5202368, hits per frag: 0.171189 processed 31000000 fragments hits: 5287724, hits per frag: 0.171101 processed 31500000 fragments hits: 5372616, hits per frag: 0.170842 processed 32000000 fragments hits: 5457410, hits per frag: 0.170991 processed 32500000 fragments hits: 5541651, hits per frag: 0.170925 processed 33000000 fragments hits: 5627175, hits per frag: 0.171047 [2019-03-14 21:17:45.386] [jointLog] [info] Computed 8127 rich equivalence classes for further processing [2019-03-14 21:17:45.386] [jointLog] [info] Counted 2818911 total reads in the equivalence classes [2019-03-14 21:17:45.397] [jointLog] [warning] 0.0111532% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 21:17:45.398] [jointLog] [warning] Only 2818911 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 21:17:45.398] [jointLog] [info] Mapping rate = 8.52724% [2019-03-14 21:17:45.398] [jointLog] [info] finished quantifyLibrary() [2019-03-14 21:17:45.398] [jointLog] [info] Starting optimizer [2019-03-14 21:17:45.403] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 21:17:45.404] [jointLog] [info] iteration = 0 | max rel diff. = 673.376 [2019-03-14 21:17:45.423] [jointLog] [info] iteration = 100 | max rel diff. = 0.123867 [2019-03-14 21:17:45.438] [jointLog] [info] iteration = 200 | max rel diff. = 0.0599776 [2019-03-14 21:17:45.454] [jointLog] [info] iteration = 300 | max rel diff. = 0.0380154 [2019-03-14 21:17:45.469] [jointLog] [info] iteration = 400 | max rel diff. = 0.0271345 [2019-03-14 21:17:45.484] [jointLog] [info] iteration = 500 | max rel diff. = 0.0249077 [2019-03-14 21:17:45.500] [jointLog] [info] iteration = 600 | max rel diff. = 0.015315 [2019-03-14 21:17:45.515] [jointLog] [info] iteration = 700 | max rel diff. = 0.0153262 [2019-03-14 21:17:45.530] [jointLog] [info] iteration = 800 | max rel diff. = 0.0135082 [2019-03-14 21:17:45.545] [jointLog] [info] iteration = 900 | max rel diff. = 0.0108925 [2019-03-14 21:17:45.560] [jointLog] [info] iteration = 1000 | max rel diff. = 0.010303 [2019-03-14 21:17:45.575] [jointLog] [info] iteration = 1100 | max rel diff. = 0.0103032 [2019-03-14 21:17:45.590] [jointLog] [info] iteration = 1200 | max rel diff. = 0.0103033 [2019-03-14 21:17:45.592] [jointLog] [info] iteration = 1217 | max rel diff. = 0.00902936 [2019-03-14 21:17:45.593] [jointLog] [info] Finished optimizer [2019-03-14 21:17:45.593] [jointLog] [info] writing output [2019-03-14 21:17:45.605] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: nb_F_K5_08/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl nb_F_K5_08/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > nb_F_K5_08/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P15-GTTTCG-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P15-GTTTCG-READ2-Sequences.txt.gz_val_2.fq.gz) -o nb_F_K5_09 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { nb_F_K5_09 } ### [ threads ] => { 23 } Logs will be written to nb_F_K5_09/logs [2019-03-14 21:17:45.920] [jointLog] [info] parsing read library format [2019-03-14 21:17:45.920] [jointLog] [info] There is 1 library. [2019-03-14 21:17:45.982] [stderrLog] [info] Loading Suffix Array [2019-03-14 21:17:45.982] [jointLog] [info] Loading Quasi index [2019-03-14 21:17:45.982] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 21:17:46.018] [stderrLog] [info] Loading Transcript Info [2019-03-14 21:17:46.028] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 21:17:46.031] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 21:17:46.033] [stderrLog] [info] Computing transcript lengths [2019-03-14 21:17:46.033] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 21:17:46.384] [jointLog] [info] done [2019-03-14 21:17:46.384] [jointLog] [info] Index contained 5838 targets [2019-03-14 21:17:46.384] [stderrLog] [info] Done loading index  processed 500001 fragments hits: 92978, hits per frag: 0.194803 processed 1000000 fragments hits: 184953, hits per frag: 0.185223 processed 1500002 fragments hits: 277415, hits per frag: 0.18554 processed 2000000 fragments hits: 370240, hits per frag: 0.191472 processed 2500001 fragments hits: 463025, hits per frag: 0.194559 processed 3000000 fragments hits: 555671, hits per frag: 0.190309 processed 3500000 fragments hits: 648556, hits per frag: 0.186832 processed 4000000 fragments hits: 741601, hits per frag: 0.187684 processed 4500002 fragments hits: 834113, hits per frag: 0.189903 processed 5000001 fragments hits: 926477, hits per frag: 0.187757 processed 5500001 fragments hits: 1018936, hits per frag: 0.186877 processed 6000001 fragments hits: 1111509, hits per frag: 0.185348 processed 6500000 fragments hits: 1204523, hits per frag: 0.185676 processed 7000000 fragments hits: 1296039, hits per frag: 0.188012 processed 7500001 fragments hits: 1388479, hits per frag: 0.186924 processed 8000001 fragments hits: 1480552, hits per frag: 0.186285 processed 8500001 fragments hits: 1572460, hits per frag: 0.187405 processed 9000000 fragments hits: 1665987, hits per frag: 0.185358 processed 9500000 fragments hits: 1758429, hits per frag: 0.186453 processed 10000000 fragments hits: 1851329, hits per frag: 0.186388 processed 10500000 fragments hits: 1944324, hits per frag: 0.185679 processed 11000000 fragments hits: 2036299, hits per frag: 0.186546 processed 11500000 fragments hits: 2130193, hits per frag: 0.186299 processed 12000000 fragments hits: 2223328, hits per frag: 0.18561 processed 12500000 fragments hits: 2316708, hits per frag: 0.186134 processed 13000000 fragments hits: 2409217, hits per frag: 0.186395 processed 13500000 fragments hits: 2502640, hits per frag: 0.185759 processed 14000000 fragments hits: 2595875, hits per frag: 0.185744 processed 14500001 fragments hits: 2688607, hits per frag: 0.185849 processed 15000000 fragments hits: 2781257, hits per frag: 0.18688 processed 15500001 fragments hits: 2873630, hits per frag: 0.186035 processed 16000000 fragments hits: 2965232, hits per frag: 0.186187 processed 16500000 fragments hits: 3058358, hits per frag: 0.185643 processed 17000000 fragments hits: 3150770, hits per frag: 0.185765 processed 17500000 fragments hits: 3243757, hits per frag: 0.185853 processed 18000000 fragments hits: 3336452, hits per frag: 0.186351 processed 18500001 fragments hits: 3428984, hits per frag: 0.186256 processed 19000000 fragments hits: 3520951, hits per frag: 0.185683 processed 19500000 fragments hits: 3612717, hits per frag: 0.186186 processed 20000000 fragments hits: 3704815, hits per frag: 0.186144 processed 20500000 fragments hits: 3796827, hits per frag: 0.185301 processed 21000000 fragments hits: 3889134, hits per frag: 0.185824 processed 21500000 fragments hits: 3981828, hits per frag: 0.185852 processed 22000000 fragments hits: 4074248, hits per frag: 0.185779 processed 22500002 fragments hits: 4165635, hits per frag: 0.185631 processed 23000000 fragments hits: 4259842, hits per frag: 0.18554 processed 23500000 fragments hits: 4351623, hits per frag: 0.185519 processed 24000000 fragments hits: 4445305, hits per frag: 0.185685 processed 24500000 fragments hits: 4537405, hits per frag: 0.185271 processed 25000000 fragments hits: 4630056, hits per frag: 0.185739 processed 25500000 fragments hits: 4721260, hits per frag: 0.185339 processed 26000000 fragments hits: 4814266, hits per frag: 0.185247 processed 26500000 fragments hits: 4907611, hits per frag: 0.186082 processed 27000001 fragments hits: 4999883, hits per frag: 0.185954 processed 27500000 fragments hits: 5092132, hits per frag: 0.185271 processed 28000000 fragments hits: 5185844, hits per frag: 0.185593 processed 28500000 fragments hits: 5278153, hits per frag: 0.185952 processed 29000000 fragments hits: 5369845, hits per frag: 0.185731 processed 29500000 fragments hits: 5461574, hits per frag: 0.185671 processed 30000000 fragments hits: 5554905, hits per frag: 0.185646 processed 30500000 fragments hits: 5647115, hits per frag: 0.185407 processed 31000000 fragments hits: 5740307, hits per frag: 0.185569 processed 31500000 fragments hits: 5832510, hits per frag: 0.185369 processed 32000000 fragments hits: 5924073, hits per frag: 0.185145 processed 32500000 fragments hits: 6017341, hits per frag: 0.185743 processed 33000000 fragments hits: 6110493, hits per frag: 0.185488 processed 33500000 fragments hits: 6203029, hits per frag: 0.185682 processed 34000000 fragments hits: 6295177, hits per frag: 0.185283 processed 34500000 fragments hits: 6387260, hits per frag: 0.18554 [2019-03-14 21:19:07.727] [jointLog] [info] Computed 7671 rich equivalence classes for further processing [2019-03-14 21:19:07.727] [jointLog] [info] Counted 3126572 total reads in the equivalence classes [2019-03-14 21:19:07.737] [jointLog] [warning] 0.0188954% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 21:19:07.738] [jointLog] [warning] Only 3126572 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 21:19:07.738] [jointLog] [info] Mapping rate = 9.05407% [2019-03-14 21:19:07.738] [jointLog] [info] finished quantifyLibrary() [2019-03-14 21:19:07.738] [jointLog] [info] Starting optimizer [2019-03-14 21:19:07.744] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 21:19:07.744] [jointLog] [info] iteration = 0 | max rel diff. = 713.347 [2019-03-14 21:19:07.765] [jointLog] [info] iteration = 100 | max rel diff. = 0.115414 [2019-03-14 21:19:07.780] [jointLog] [info] iteration = 200 | max rel diff. = 0.0574996 [2019-03-14 21:19:07.796] [jointLog] [info] iteration = 300 | max rel diff. = 0.0433144 [2019-03-14 21:19:07.811] [jointLog] [info] iteration = 400 | max rel diff. = 0.0297571 [2019-03-14 21:19:07.826] [jointLog] [info] iteration = 500 | max rel diff. = 0.0247571 [2019-03-14 21:19:07.842] [jointLog] [info] iteration = 600 | max rel diff. = 0.019155 [2019-03-14 21:19:07.859] [jointLog] [info] iteration = 700 | max rel diff. = 0.0155012 [2019-03-14 21:19:07.877] [jointLog] [info] iteration = 800 | max rel diff. = 0.0129266 [2019-03-14 21:19:07.895] [jointLog] [info] iteration = 900 | max rel diff. = 0.0101357 [2019-03-14 21:19:07.913] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0101356 [2019-03-14 21:19:07.932] [jointLog] [info] iteration = 1100 | max rel diff. = 0.0101355 [2019-03-14 21:19:07.940] [jointLog] [info] iteration = 1143 | max rel diff. = 0.00896338 [2019-03-14 21:19:07.940] [jointLog] [info] Finished optimizer [2019-03-14 21:19:07.940] [jointLog] [info] writing output [2019-03-14 21:19:07.951] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: nb_F_K5_09/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl nb_F_K5_09/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > nb_F_K5_09/quant.sf.genes