--- title: "08-gene-files" output: html_document --- Getting fasta files from the Genome annotation as opposed to version 5. Will use getfasta.. ```{bash} cd ../data wget --no-check-certificate \ https://gannet.fish.washington.edu/Atumefaciens/20190928_Pgenerosa_v074.a4_gensas_annotation/Panopea-generosa-vv0.74.a4.repeat_region.gff3 ``` ```{bash} head ../genome/Panopea-generosa-v1.0.fa ``` ```{bash} head ../data/Panopea-generosa-vv0.74.a4.mRNA.gff3 ``` ```{bash} /home/shared/bedtools2/bin/bedtools getfasta \ -fi ../genome/Panopea-generosa-v1.0.fa \ -bed ../data/Panopea-generosa-vv0.74.a4.mRNA.gff3 \ -fo ../analyses/Pg_v0.74.a4.mRNA.fa ```