FastQCFastQC Report
Mon 7 Feb 2022
SRR12227931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12227931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181549
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10230.5634842384149734TruSeq Adapter, Index 5 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4620.2544767528325686No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC3350.18452318657772834TruSeq Adapter, Index 5 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)