FastQCFastQC Report
Mon 7 Feb 2022
SRR12226692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12226692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13976789
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC4210983.0128379272234844TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC1449161.0368332812350534TruSeq Adapter, Index 6 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT439330.31432827668787156TruSeq Adapter, Index 6 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204190.14609221044976783No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGCCGT160890.11511227650356601TruSeq Adapter, Index 6 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)