FastQCFastQC Report
Mon 7 Feb 2022
SRR12218870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12218870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59474116
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC25314854.256448300971805TruSeq Adapter, Index 12 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2980620.5011625561614064TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT782030.13149081526491288No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN649360.10918363208626758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)