FastQCFastQC Report
Mon 7 Feb 2022
SRR12218869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12218869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55671619
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG24939704.47978708864206Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT8338371.4977775300553051Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1725490.3099406898872476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN713990.12825026698073932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)