FastQCFastQC Report
Mon 7 Feb 2022
SRR12218869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12218869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55671619
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC24118084.332203811065742TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC7842531.408712399759741TruSeq Adapter, Index 6 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1563030.2807588548843891No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT1555930.27948351924164444TruSeq Adapter, Index 6 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN693370.12454640487462741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)