FastQCFastQC Report
Mon 7 Feb 2022
SRR12207406_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12207406_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91451743
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2572550.2813013634961556Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2112310.23097536806925595Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1075660.11762050287002185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)