FastQCFastQC Report
Mon 7 Feb 2022
SRR12207405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12207405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62928954
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT3311500.5262283558693825TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1553210.2468196118435403TruSeq Adapter, Index 15 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800600.12722283608909182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)