Version MEGAN Community Edition (version 6.21.5, built 5 May 2021) Author(s) Daniel H. Huson Copyright (C) 2021 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Java version: 14.0.1 Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,302,807 Loading ncbi.tre: 2,302,811 Loading ec.map: 8,081 Loading ec.tre: 8,085 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 13,894 Loading interpro2go.tre: 28,869 Loading seed.map: 979 Loading seed.tre: 980 Meganizing: mcap_READ1_all.blastx.daa Meganizing init Annotating DAA file using FAST mode (accession database and first accession per line) Annotating references 10% 20% 30% 100% (1678.4s) Writing 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2.4s) Binning reads Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads Analyzing alignments 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (35401.1s) Total reads: 73,340,733 With hits: 73,340,733 Alignments: 19,322,417,083 Assig. Taxonomy: 73,300,688 Assig. SEED: 1,785,954 Assig. EGGNOG: 590,469 Assig. GTDB: 4,133,207 Assig. EC: 18,288,401 Assig. INTERPRO2GO: 17,020,057 MinSupport set to: 36670 Binning reads Applying min-support & disabled filter to Taxonomy... 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (0.7s) Min-supp. changes: 5,654 Binning reads Applying min-support & disabled filter to GTDB... 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (448.8s) Min-supp. changes: 6,168 Binning reads Writing classification tables 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (1279.5s) Binning reads Syncing 100% (0.2s) Class. Taxonomy: 84 Class. SEED: 337 Class. EGGNOG: 814 Class. GTDB: 52 Class. EC: 1,522 Class. INTERPRO2GO: 7,371 Meganizing: mcap_READ2_all.blastx.daa Meganizing init Annotating DAA file using FAST mode (accession database and first accession per line) Annotating references 10% 20% 30% 100% (346.4s) Writing 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2.5s) Binning reads Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads Analyzing alignments 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (28915.2s) Total reads: 69,987,383 With hits: 69,987,383 Alignments: 15,884,214,571 Assig. Taxonomy: 69,948,494 Assig. SEED: 1,677,686 Assig. EGGNOG: 488,655 Assig. GTDB: 3,676,452 Assig. EC: 17,345,279 Assig. INTERPRO2GO: 16,017,324 MinSupport set to: 34993 Binning reads Applying min-support & disabled filter to Taxonomy... 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (0.6s) Min-supp. changes: 7,478 Binning reads Applying min-support & disabled filter to GTDB... 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (564.6s) Min-supp. changes: 7,506 Binning reads Writing classification tables 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (1357.1s) Binning reads Syncing 100% (0.1s) Class. Taxonomy: 92 Class. SEED: 345 Class. EGGNOG: 807 Class. GTDB: 50 Class. EC: 1,547 Class. INTERPRO2GO: 7,872 Total time: 70,006s Peak memory: 69.8 of 195.3G --------------------- Total runtime was: 70013 seconds