______ ____ ____ ____ ____ ______ __ __ | || \ | || \ | || || | | | || D ) | | | _ | | | | || | | |_| |_|| / | | | | | | | |_| |_|| ~ | | | | \ | | | | | | | | | |___, | | | | . \ | | | | | | | | | | | |__| |__|\_||____||__|__||____| |__| |____/ Left read files: $VAR1 = [ '4R041-L6-P01-AGTCAA-READ1-Sequences.txt.gz', '4R041-L6-P02-CTTGTA-READ1-Sequences.txt.gz', '4R041-L6-P03-GTTTCG-READ1-Sequences.txt.gz', '4R041-L6-P04-ATCACG-READ1-Sequences.txt.gz', '4R041-L6-P05-TAGCTT-READ1-Sequences.txt.gz', '4R041-L6-P06-AGTTCC-READ1-Sequences.txt.gz', '4R041-L6-P07-ATGTCA-READ1-Sequences.txt.gz', '4R041-L6-P08-ACTGAT-READ1-Sequences.txt.gz', '4R041-L6-P09-GGCTAC-READ1-Sequences.txt.gz', '4R041-L6-P10-GTCCGC-READ1-Sequences.txt.gz', '4R041-L6-P11-GAGTGG-READ1-Sequences.txt.gz', '4R041-L6-P12-GCCAAT-READ1-Sequences.txt.gz', '4R041-L6-PrNotRecog-READ1-Sequences.txt.gz' ]; Right read files: $VAR1 = [ '4R041-L6-P01-AGTCAA-READ2-Sequences.txt.gz', '4R041-L6-P02-CTTGTA-READ2-Sequences.txt.gz', '4R041-L6-P03-GTTTCG-READ2-Sequences.txt.gz', '4R041-L6-P04-ATCACG-READ2-Sequences.txt.gz', '4R041-L6-P05-TAGCTT-READ2-Sequences.txt.gz', '4R041-L6-P06-AGTTCC-READ2-Sequences.txt.gz', '4R041-L6-P07-ATGTCA-READ2-Sequences.txt.gz', '4R041-L6-P08-ACTGAT-READ2-Sequences.txt.gz', '4R041-L6-P09-GGCTAC-READ2-Sequences.txt.gz', '4R041-L6-P10-GTCCGC-READ2-Sequences.txt.gz', '4R041-L6-P11-GAGTGG-READ2-Sequences.txt.gz', '4R041-L6-P12-GCCAAT-READ2-Sequences.txt.gz', '4R041-L6-PrNotRecog-READ2-Sequences.txt.gz' ]; Trinity version: Trinity-v2.6.5 ** NOTE: Latest version of Trinity is Trinity-v2.6.6, and can be obtained at: https://github.com/trinityrnaseq/trinityrnaseq/releases Monday, April 2, 2018: 16:02:24 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /home/shared/trinityrnaseq-Trinity-v2.6.5/util/support_scripts/ExitTester.jar 0 Monday, April 2, 2018: 16:02:24 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /home/shared/trinityrnaseq-Trinity-v2.6.5/util/support_scripts/ExitTester.jar 1 Monday, April 2, 2018: 16:02:24 CMD: mkdir -p /media/sam/4TB_toshiba/montipora/20180402_trinity/chrysalis ---------------------------------------------------------------------------------- -------------- Trinity Phase 1: Clustering of RNA-Seq Reads --------------------- ---------------------------------------------------------------------------------- --------------------------------------------------------------- ------------ In silico Read Normalization --------------------- -- (Removing Excess Reads Beyond 50 Coverage -- --------------------------------------------------------------- # running normalization on reads: $VAR1 = [ [ '/media/sam/4TB_toshiba/montipora/4R041-L6-P01-AGTCAA-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P02-CTTGTA-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P03-GTTTCG-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P04-ATCACG-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P05-TAGCTT-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P06-AGTTCC-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P07-ATGTCA-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P08-ACTGAT-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P09-GGCTAC-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P10-GTCCGC-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P11-GAGTGG-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P12-GCCAAT-READ1-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-PrNotRecog-READ1-Sequences.txt.gz' ], [ '/media/sam/4TB_toshiba/montipora/4R041-L6-P01-AGTCAA-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P02-CTTGTA-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P03-GTTTCG-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P04-ATCACG-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P05-TAGCTT-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P06-AGTTCC-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P07-ATGTCA-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P08-ACTGAT-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P09-GGCTAC-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P10-GTCCGC-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P11-GAGTGG-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-P12-GCCAAT-READ2-Sequences.txt.gz', '/media/sam/4TB_toshiba/montipora/4R041-L6-PrNotRecog-READ2-Sequences.txt.gz' ] ]; Monday, April 2, 2018: 16:02:24 CMD: /home/shared/trinityrnaseq-Trinity-v2.6.5/util/insilico_read_normalization.pl --seqType fq --JM 90G --max_cov 50 --min_cov 1 --CPU 24 --output /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization --max_pct_stdev 10000 --left /media/sam/4TB_toshiba/montipora/4R041-L6-P01-AGTCAA-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P02-CTTGTA-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P03-GTTTCG-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P04-ATCACG-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P05-TAGCTT-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P06-AGTTCC-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P07-ATGTCA-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P08-ACTGAT-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P09-GGCTAC-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P10-GTCCGC-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P11-GAGTGG-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P12-GCCAAT-READ1-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-PrNotRecog-READ1-Sequences.txt.gz --right /media/sam/4TB_toshiba/montipora/4R041-L6-P01-AGTCAA-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P02-CTTGTA-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P03-GTTTCG-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P04-ATCACG-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P05-TAGCTT-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P06-AGTTCC-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P07-ATGTCA-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P08-ACTGAT-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P09-GGCTAC-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P10-GTCCGC-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P11-GAGTGG-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-P12-GCCAAT-READ2-Sequences.txt.gz,/media/sam/4TB_toshiba/montipora/4R041-L6-PrNotRecog-READ2-Sequences.txt.gz --pairs_together --PARALLEL_STATS Converting input files. (both directions in parallel)CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P01-AGTCAA-READ1-Sequences.txt.gz) >> left.fa CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P01-AGTCAA-READ2-Sequences.txt.gz) >> right.fa CMD finished (210 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P02-CTTGTA-READ1-Sequences.txt.gz) >> left.fa CMD finished (290 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P02-CTTGTA-READ2-Sequences.txt.gz) >> right.fa CMD finished (376 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P03-GTTTCG-READ1-Sequences.txt.gz) >> left.fa CMD finished (391 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P03-GTTTCG-READ2-Sequences.txt.gz) >> right.fa CMD finished (237 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P04-ATCACG-READ1-Sequences.txt.gz) >> left.fa CMD finished (286 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P04-ATCACG-READ2-Sequences.txt.gz) >> right.fa CMD finished (296 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P05-TAGCTT-READ1-Sequences.txt.gz) >> left.fa CMD finished (283 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P05-TAGCTT-READ2-Sequences.txt.gz) >> right.fa CMD finished (323 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P06-AGTTCC-READ1-Sequences.txt.gz) >> left.fa CMD finished (387 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P06-AGTTCC-READ2-Sequences.txt.gz) >> right.fa CMD finished (465 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P07-ATGTCA-READ1-Sequences.txt.gz) >> left.fa CMD finished (497 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P07-ATGTCA-READ2-Sequences.txt.gz) >> right.fa CMD finished (286 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P08-ACTGAT-READ1-Sequences.txt.gz) >> left.fa CMD finished (356 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P08-ACTGAT-READ2-Sequences.txt.gz) >> right.fa CMD finished (422 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P09-GGCTAC-READ1-Sequences.txt.gz) >> left.fa CMD finished (511 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P09-GGCTAC-READ2-Sequences.txt.gz) >> right.fa CMD finished (400 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P10-GTCCGC-READ1-Sequences.txt.gz) >> left.fa CMD finished (129 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P11-GAGTGG-READ1-Sequences.txt.gz) >> left.fa CMD finished (398 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P10-GTCCGC-READ2-Sequences.txt.gz) >> right.fa CMD finished (212 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P11-GAGTGG-READ2-Sequences.txt.gz) >> right.fa CMD finished (476 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P12-GCCAAT-READ1-Sequences.txt.gz) >> left.fa CMD finished (424 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-PrNotRecog-READ1-Sequences.txt.gz) >> left.fa CMD finished (8 seconds) CMD finished (441 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-P12-GCCAAT-READ2-Sequences.txt.gz) >> right.fa CMD finished (244 seconds) CMD: seqtk-trinity seq -A <(gunzip -c /media/sam/4TB_toshiba/montipora/4R041-L6-PrNotRecog-READ2-Sequences.txt.gz) >> right.fa CMD finished (82 seconds) CMD: touch left.fa.ok CMD finished (0 seconds) CMD: touch right.fa.ok CMD finished (0 seconds) Done converting input files.CMD: cat left.fa right.fa > both.fa CMD finished (4499 seconds) CMD: touch both.fa.ok ------------------------------------------- ----------- Jellyfish -------------------- -- (building a k-mer catalog from reads) -- ------------------------------------------- CMD finished (0 seconds) CMD: jellyfish count -t 24 -m 25 -s 100000000 --canonical both.fa CMD finished (3825 seconds) CMD: jellyfish histo -t 24 -o jellyfish.K25.min2.kmers.fa.histo mer_counts.jf CMD finished (144 seconds) CMD: jellyfish dump -L 2 mer_counts.jf > jellyfish.K25.min2.kmers.fa CMD finished (957 seconds) CMD: touch jellyfish.K25.min2.kmers.fa.success CMD finished (0 seconds) CMD: /home/shared/trinityrnaseq-Trinity-v2.6.5/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads left.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 12 --DS > left.fa.K25.stats CMD: /home/shared/trinityrnaseq-Trinity-v2.6.5/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads right.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 12 --DS > right.fa.K25.stats -reading Kmer occurrences...-reading Kmer occurrences... done parsing 893033817 Kmers, 893033817 added, taking 1332 seconds. done parsing 893033817 Kmers, 893033817 added, taking 1350 seconds. STATS_GENERATION_TIME: 6541 seconds. STATS_GENERATION_TIME: 6613 seconds. CMD finished (8083 seconds) CMD finished (8146 seconds) CMD: touch left.fa.K25.stats.ok CMD finished (0 seconds) CMD: touch right.fa.K25.stats.ok -sorting each stats file by read name. CMD finished (0 seconds) CMD: /usr/bin/sort --parallel=24 -k5,5 -T . -S 45G left.fa.K25.stats > left.fa.K25.stats.sort CMD: /usr/bin/sort --parallel=24 -k5,5 -T . -S 45G right.fa.K25.stats > right.fa.K25.stats.sort CMD finished (6540 seconds) CMD finished (6605 seconds) CMD: touch left.fa.K25.stats.sort.ok CMD finished (0 seconds) CMD: touch right.fa.K25.stats.sort.ok CMD finished (0 seconds) CMD: /home/shared/trinityrnaseq-Trinity-v2.6.5/util/..//util/support_scripts//nbkc_merge_left_right_stats.pl --left left.fa.K25.stats.sort --right right.fa.K25.stats.sort --sorted > pairs.K25.stats -opening left.fa.K25.stats.sort -opening right.fa.K25.stats.sort -done opening files. CMD finished (3411 seconds) CMD: touch pairs.K25.stats.ok CMD finished (0 seconds) CMD: /home/shared/trinityrnaseq-Trinity-v2.6.5/util/..//util/support_scripts//nbkc_normalize.pl --stats_file pairs.K25.stats --max_cov 50 --min_cov 1 --max_pct_stdev 10000 > pairs.K25.stats.C50.pctSD10000.accs 63277282 / 390534533 = 16.20% reads selected during normalization. 0 / 390534533 = 0.00% reads discarded as likely aberrant based on coverage profiles. 0 / 390534533 = 0.00% reads missing kmer coverage (N chars included?). 0 / 390534533 = 0.00% reads discarded as below minimum coverage threshold=1 CMD finished (682 seconds) CMD: touch pairs.K25.stats.C50.pctSD10000.accs.ok CMD finished (0 seconds) CMD: touch /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/4R041-L6-P01-AGTCAA-READ1-Sequences.txt.gz_ext_all_reads.normalized_K25_maxC50_minC1_pctSD10000.fq.ok CMD finished (0 seconds) CMD: touch /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/4R041-L6-P01-AGTCAA-READ2-Sequences.txt.gz_ext_all_reads.normalized_K25_maxC50_minC1_pctSD10000.fq.ok CMD finished (0 seconds) CMD: ln -sf /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/4R041-L6-P01-AGTCAA-READ1-Sequences.txt.gz_ext_all_reads.normalized_K25_maxC50_minC1_pctSD10000.fq left.norm.fq CMD finished (0 seconds) CMD: ln -sf /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/4R041-L6-P01-AGTCAA-READ2-Sequences.txt.gz_ext_all_reads.normalized_K25_maxC50_minC1_pctSD10000.fq right.norm.fq -removing tmp dir /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/tmp_normalized_reads CMD finished (0 seconds) Normalization complete. See outputs: /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/4R041-L6-P01-AGTCAA-READ1-Sequences.txt.gz_ext_all_reads.normalized_K25_maxC50_minC1_pctSD10000.fq /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/4R041-L6-P01-AGTCAA-READ2-Sequences.txt.gz_ext_all_reads.normalized_K25_maxC50_minC1_pctSD10000.fq Tuesday, April 3, 2018: 03:29:37 CMD: touch /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/normalization.ok Converting input files. (in parallel)Tuesday, April 3, 2018: 03:29:37 CMD: cat /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/left.norm.fq | seqtk-trinity seq -A - >> left.fa Tuesday, April 3, 2018: 03:29:37 CMD: cat /media/sam/4TB_toshiba/montipora/20180402_trinity/insilico_read_normalization/right.norm.fq | seqtk-trinity seq -A - >> right.fa Tuesday, April 3, 2018: 03:43:57 CMD: touch left.fa.ok Tuesday, April 3, 2018: 03:43:58 CMD: touch right.fa.ok Tuesday, April 3, 2018: 03:43:58 CMD: touch left.fa.ok right.fa.ok Tuesday, April 3, 2018: 03:43:58 CMD: cat left.fa right.fa > /media/sam/4TB_toshiba/montipora/20180402_trinity/both.fa Tuesday, April 3, 2018: 03:48:19 CMD: touch /media/sam/4TB_toshiba/montipora/20180402_trinity/both.fa.ok ------------------------------------------- ----------- Jellyfish -------------------- -- (building a k-mer catalog from reads) -- ------------------------------------------- * Running CMD: jellyfish count -t 24 -m 25 -s 11227976716 --canonical /media/sam/4TB_toshiba/montipora/20180402_trinity/both.fa * Running CMD: jellyfish dump -L 1 mer_counts.jf > jellyfish.kmers.fa * Running CMD: jellyfish histo -t 24 -o jellyfish.kmers.fa.histo mer_counts.jf ---------------------------------------------- --------------- Inchworm --------------------- -- (Linear contig construction from k-mers) -- ---------------------------------------------- * Running CMD: /home/shared/trinityrnaseq-Trinity-v2.6.5/Inchworm/bin//inchworm --kmers jellyfish.kmers.fa --run_inchworm -K 25 -L 25 --monitor 1 --DS --num_threads 6 --PARALLEL_IWORM > /media/sam/4TB_toshiba/montipora/20180402_trinity/inchworm.K25.L25.DS.fa.tmp Kmer length set to: 25 Min assembly length set to: 25 Monitor turned on, set to: 1 double stranded mode set setting number of threads to: 6 -setting parallel iworm mode. -reading Kmer occurrences... [0M] Kmers parsed. [1M] Kmers parsed. [2M] Kmers parsed. [3M] Kmers parsed. [4M] Kmers parsed. [5M] Kmers parsed. [6M] Kmers parsed. [7M] Kmers parsed. [7M] Kmers parsed. [8M] Kmers parsed. [9M] Kmers parsed. [10M] Kmers parsed. [11M] Kmers parsed. [12M] Kmers parsed. [13M] Kmers parsed. [13M] Kmers parsed. [14M] Kmers parsed. [15M] Kmers parsed. [16M] Kmers parsed. [17M] Kmers parsed. [18M] Kmers parsed. [19M] Kmers parsed. [19M] Kmers parsed. [20M] Kmers parsed. [21M] Kmers parsed. [22M] Kmers parsed. [23M] Kmers parsed. [24M] Kmers parsed. [25M] Kmers parsed. [26M] Kmers parsed. [27M] Kmers parsed. [27M] Kmers parsed. [28M] Kmers parsed. [29M] Kmers parsed. [30M] Kmers parsed. [31M] Kmers parsed. [32M] Kmers parsed. [33M] Kmers parsed. [34M] Kmers parsed. [34M] Kmers parsed. [35M] Kmers parsed. [36M] Kmers parsed. [37M] Kmers parsed. [38M] Kmers parsed. [39M] Kmers parsed. [39M] Kmers parsed. [40M] Kmers parsed. [41M] Kmers parsed. [41M] Kmers parsed. [42M] Kmers parsed. 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[84M] Kmers parsed. [85M] Kmers parsed. [85M] Kmers parsed. [86M] Kmers parsed. [87M] Kmers parsed. [88M] Kmers parsed. [88M] Kmers parsed. [89M] Kmers parsed. [90M] Kmers parsed. [91M] Kmers parsed. [91M] Kmers parsed. [92M] Kmers parsed. [93M] Kmers parsed. [93M] Kmers parsed. [94M] Kmers parsed. [95M] Kmers parsed. [95M] Kmers parsed. [96M] Kmers parsed. [97M] Kmers parsed. [98M] Kmers parsed. [98M] Kmers parsed. [99M] Kmers parsed. [100M] Kmers parsed. [100M] Kmers parsed. [101M] Kmers parsed. [102M] Kmers parsed. [103M] Kmers parsed. [104M] Kmers parsed. [105M] Kmers parsed. [106M] Kmers parsed. [106M] Kmers parsed. [107M] Kmers parsed. [108M] Kmers parsed. [109M] Kmers parsed. [109M] Kmers parsed. [110M] Kmers parsed. [111M] Kmers parsed. [112M] Kmers parsed. [112M] Kmers parsed. [113M] Kmers parsed. [114M] Kmers parsed. [115M] Kmers parsed. [115M] Kmers parsed. [116M] Kmers parsed. [117M] Kmers parsed. [117M] Kmers parsed. [118M] Kmers parsed. [119M] Kmers parsed. [120M] Kmers parsed. [120M] Kmers parsed. [121M] Kmers parsed. [122M] Kmers parsed. [122M] Kmers parsed. [123M] Kmers parsed. [124M] Kmers parsed. [124M] Kmers parsed. [125M] Kmers parsed. [126M] Kmers parsed. [127M] Kmers parsed. [127M] Kmers parsed. [128M] Kmers parsed. [129M] Kmers parsed. [129M] Kmers parsed. [130M] Kmers parsed. [131M] Kmers parsed. [131M] Kmers parsed. [132M] Kmers parsed. [133M] Kmers parsed. [133M] Kmers parsed. [134M] Kmers parsed. [135M] Kmers parsed. [136M] Kmers parsed. [137M] Kmers parsed. [137M] Kmers parsed. [138M] Kmers parsed. [139M] Kmers parsed. [139M] Kmers parsed. [140M] Kmers parsed. [141M] Kmers parsed. [142M] Kmers parsed. [142M] Kmers parsed. [143M] Kmers parsed. [144M] Kmers parsed. [144M] Kmers parsed. [145M] Kmers parsed. [145M] Kmers parsed. [146M] Kmers parsed. [147M] Kmers parsed. [147M] Kmers parsed. [148M] Kmers parsed. [149M] Kmers parsed. [150M] Kmers parsed. [150M] Kmers parsed. [151M] Kmers parsed. [151M] Kmers parsed. 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[184M] Kmers parsed. [185M] Kmers parsed. [185M] Kmers parsed. [186M] Kmers parsed. [187M] Kmers parsed. [187M] Kmers parsed. [188M] Kmers parsed. [189M] Kmers parsed. [189M] Kmers parsed. [190M] Kmers parsed. [190M] Kmers parsed. [191M] Kmers parsed. [192M] Kmers parsed. [192M] Kmers parsed. [193M] Kmers parsed. [193M] Kmers parsed. [194M] Kmers parsed. [195M] Kmers parsed. [195M] Kmers parsed. [196M] Kmers parsed. [197M] Kmers parsed. [197M] Kmers parsed. [198M] Kmers parsed. [198M] Kmers parsed. [199M] Kmers parsed. [200M] Kmers parsed. [200M] Kmers parsed. [201M] Kmers parsed. [202M] Kmers parsed. [203M] Kmers parsed. [204M] Kmers parsed. [204M] Kmers parsed. [205M] Kmers parsed. [206M] Kmers parsed. [206M] Kmers parsed. [207M] Kmers parsed. [208M] Kmers parsed. [209M] Kmers parsed. [209M] Kmers parsed. [210M] Kmers parsed. [211M] Kmers parsed. [212M] Kmers parsed. [212M] Kmers parsed. [213M] Kmers parsed. [214M] Kmers parsed. [214M] Kmers parsed. [215M] Kmers parsed. 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[465M] Kmers parsed. [465M] Kmers parsed. [466M] Kmers parsed. [467M] Kmers parsed. [467M] Kmers parsed. [468M] Kmers parsed. [469M] Kmers parsed. [469M] Kmers parsed. [470M] Kmers parsed. [471M] Kmers parsed. [471M] Kmers parsed. [472M] Kmers parsed. [473M] Kmers parsed. [474M] Kmers parsed. [474M] Kmers parsed. [475M] Kmers parsed. [476M] Kmers parsed. [476M] Kmers parsed. [477M] Kmers parsed. [478M] Kmers parsed. [479M] Kmers parsed. [479M] Kmers parsed. [480M] Kmers parsed. [481M] Kmers parsed. [482M] Kmers parsed. [482M] Kmers parsed. [483M] Kmers parsed. [484M] Kmers parsed. [485M] Kmers parsed. [485M] Kmers parsed. [486M] Kmers parsed. [487M] Kmers parsed. [488M] Kmers parsed. [488M] Kmers parsed. [489M] Kmers parsed. [489M] Kmers parsed. [490M] Kmers parsed. [491M] Kmers parsed. [492M] Kmers parsed. [492M] Kmers parsed. [493M] Kmers parsed. [494M] Kmers parsed. [494M] Kmers parsed. [495M] Kmers parsed. [496M] Kmers parsed. [496M] Kmers parsed. [497M] Kmers parsed. [498M] Kmers parsed. [499M] Kmers parsed. [499M] Kmers parsed. [500M] Kmers parsed. [500M] Kmers parsed. [501M] Kmers parsed. [502M] Kmers parsed. [503M] Kmers parsed. [503M] Kmers parsed. [504M] Kmers parsed. [505M] Kmers parsed. [505M] Kmers parsed. [506M] Kmers parsed. [507M] Kmers parsed. [507M] Kmers parsed. [508M] Kmers parsed. [509M] Kmers parsed. [509M] Kmers parsed. [510M] Kmers parsed. [511M] Kmers parsed. [511M] Kmers parsed. [512M] Kmers parsed. [513M] Kmers parsed. [513M] Kmers parsed. [514M] Kmers parsed. [515M] Kmers parsed. [515M] Kmers parsed. [516M] Kmers parsed. [517M] Kmers parsed. [517M] Kmers parsed. [518M] Kmers parsed. [519M] Kmers parsed. [519M] Kmers parsed. [520M] Kmers parsed. [521M] Kmers parsed. [521M] Kmers parsed. [522M] Kmers parsed. [523M] Kmers parsed. [524M] Kmers parsed. [524M] Kmers parsed. [525M] Kmers parsed. [526M] Kmers parsed. [526M] Kmers parsed. [527M] Kmers parsed. [528M] Kmers parsed. [528M] Kmers parsed. [529M] Kmers parsed. [530M] Kmers parsed. [530M] Kmers parsed. [531M] Kmers parsed. [532M] Kmers parsed. [532M] Kmers parsed. [533M] Kmers parsed. [534M] Kmers parsed. [534M] Kmers parsed. [535M] Kmers parsed. [536M] Kmers parsed. [536M] Kmers parsed. [537M] Kmers parsed. [538M] Kmers parsed. [539M] Kmers parsed. [539M] Kmers parsed. [540M] Kmers parsed. [541M] Kmers parsed. [541M] Kmers parsed. [542M] Kmers parsed. [543M] Kmers parsed. [543M] Kmers parsed. [544M] Kmers parsed. [545M] Kmers parsed. [546M] Kmers parsed. [546M] Kmers parsed. [547M] Kmers parsed. [548M] Kmers parsed. [549M] Kmers parsed. [549M] Kmers parsed. [550M] Kmers parsed. [551M] Kmers parsed. [551M] Kmers parsed. [552M] Kmers parsed. [553M] Kmers parsed. [553M] Kmers parsed. [554M] Kmers parsed. [555M] Kmers parsed. [556M] Kmers parsed. [556M] Kmers parsed. [557M] Kmers parsed. [558M] Kmers parsed. [559M] Kmers parsed. [559M] Kmers parsed. [560M] Kmers parsed. [560M] Kmers parsed. [561M] Kmers parsed. [562M] Kmers parsed. [563M] Kmers parsed. [563M] Kmers parsed. [564M] Kmers parsed. [565M] Kmers parsed. [565M] Kmers parsed. [566M] Kmers parsed. [567M] Kmers parsed. [567M] Kmers parsed. [568M] Kmers parsed. [569M] Kmers parsed. [569M] Kmers parsed. [570M] Kmers parsed. [571M] Kmers parsed. [571M] Kmers parsed. [572M] Kmers parsed. [573M] Kmers parsed. [573M] Kmers parsed. [574M] Kmers parsed. [575M] Kmers parsed. [576M] Kmers parsed. [576M] Kmers parsed. [577M] Kmers parsed. [578M] Kmers parsed. [578M] Kmers parsed. [579M] Kmers parsed. [580M] Kmers parsed. [580M] Kmers parsed. [581M] Kmers parsed. [582M] Kmers parsed. [582M] Kmers parsed. [583M] Kmers parsed. [584M] Kmers parsed. [585M] Kmers parsed. [585M] Kmers parsed. [586M] Kmers parsed. [587M] Kmers parsed. [587M] Kmers parsed. [588M] Kmers parsed. [589M] Kmers parsed. [589M] Kmers parsed. [590M] Kmers parsed. [591M] Kmers parsed. [591M] Kmers parsed. [592M] Kmers parsed. [593M] Kmers parsed. [593M] Kmers parsed. [594M] Kmers parsed. [595M] Kmers parsed. [595M] Kmers parsed. [596M] Kmers parsed. [597M] Kmers parsed. [598M] Kmers parsed. [598M] Kmers parsed. [599M] Kmers parsed. [600M] Kmers parsed. [601M] Kmers parsed. [601M] Kmers parsed. [602M] Kmers parsed. [603M] Kmers parsed. [603M] Kmers parsed. [604M] Kmers parsed. [605M] Kmers parsed. [605M] Kmers parsed. [606M] Kmers parsed. [607M] Kmers parsed. [607M] Kmers parsed. [608M] Kmers parsed. [609M] Kmers parsed. [609M] Kmers parsed. [610M] Kmers parsed. [611M] Kmers parsed. [612M] Kmers parsed. [612M] Kmers parsed. [613M] Kmers parsed. [614M] Kmers parsed. [614M] Kmers parsed. [615M] Kmers parsed. [616M] Kmers parsed. [616M] Kmers parsed. [617M] Kmers parsed. [618M] Kmers parsed. [618M] Kmers parsed. [619M] Kmers parsed. [620M] Kmers parsed. [620M] Kmers parsed. [621M] Kmers parsed. [622M] Kmers parsed. [622M] Kmers parsed. [623M] Kmers parsed. [624M] Kmers parsed. [624M] Kmers parsed. [625M] Kmers parsed. [626M] Kmers parsed. [626M] Kmers parsed. [627M] Kmers parsed. [628M] Kmers parsed. [628M] Kmers parsed. [629M] Kmers parsed. [630M] Kmers parsed. [631M] Kmers parsed. [631M] Kmers parsed. [632M] Kmers parsed. [633M] Kmers parsed. [633M] Kmers parsed. [634M] Kmers parsed. [635M] Kmers parsed. [635M] Kmers parsed. [636M] Kmers parsed. [637M] Kmers parsed. [637M] Kmers parsed. [638M] Kmers parsed. [639M] Kmers parsed. [639M] Kmers parsed. [640M] Kmers parsed. [641M] Kmers parsed. [641M] Kmers parsed. [642M] Kmers parsed. [643M] Kmers parsed. [644M] Kmers parsed. [644M] Kmers parsed. [645M] Kmers parsed. [646M] Kmers parsed. [647M] Kmers parsed. [647M] Kmers parsed. [648M] Kmers parsed. [649M] Kmers parsed. [649M] Kmers parsed. [650M] Kmers parsed. [651M] Kmers parsed. [651M] Kmers parsed. [652M] Kmers parsed. [653M] Kmers parsed. [654M] Kmers parsed. [654M] Kmers parsed. [655M] Kmers parsed. [656M] Kmers parsed. [656M] Kmers parsed. [657M] Kmers parsed. [658M] Kmers parsed. [659M] Kmers parsed. [659M] Kmers parsed. [660M] Kmers parsed. [661M] Kmers parsed. [661M] Kmers parsed. [662M] Kmers parsed. [663M] Kmers parsed. [664M] Kmers parsed. [664M] Kmers parsed. [665M] Kmers parsed. [666M] Kmers parsed. [666M] Kmers parsed. [667M] Kmers parsed. [668M] Kmers parsed. [668M] Kmers parsed. [669M] Kmers parsed. [670M] Kmers parsed. [671M] Kmers parsed. [671M] Kmers parsed. [672M] Kmers parsed. [673M] Kmers parsed. [673M] Kmers parsed. [674M] Kmers parsed. [675M] Kmers parsed. [676M] Kmers parsed. [676M] Kmers parsed. [677M] Kmers parsed. [678M] Kmers parsed. [679M] Kmers parsed. [679M] Kmers parsed. [680M] Kmers parsed. [681M] Kmers parsed. [681M] Kmers parsed. [682M] Kmers parsed. [683M] Kmers parsed. [684M] Kmers parsed. [684M] Kmers parsed. [685M] Kmers parsed. [686M] Kmers parsed. [687M] Kmers parsed. [687M] Kmers parsed. [688M] Kmers parsed. [689M] Kmers parsed. [690M] Kmers parsed. [690M] Kmers parsed. [691M] Kmers parsed. [692M] Kmers parsed. [692M] Kmers parsed. [693M] Kmers parsed. [694M] Kmers parsed. [695M] Kmers parsed. [696M] Kmers parsed. [696M] Kmers parsed. [697M] Kmers parsed. [698M] Kmers parsed. [699M] Kmers parsed. [699M] Kmers parsed. [700M] Kmers parsed. [701M] Kmers parsed. [701M] Kmers parsed. [702M] Kmers parsed. [703M] Kmers parsed. [703M] Kmers parsed. [704M] Kmers parsed. [705M] Kmers parsed. [706M] Kmers parsed. [706M] Kmers parsed. [707M] Kmers parsed. [708M] Kmers parsed. [709M] Kmers parsed. [709M] Kmers parsed. [710M] Kmers parsed. [711M] Kmers parsed. [711M] Kmers parsed. [712M] Kmers parsed. [712M] Kmers parsed. [713M] Kmers parsed. [714M] Kmers parsed. [714M] Kmers parsed. [715M] Kmers parsed. [716M] Kmers parsed. [717M] Kmers parsed. [717M] Kmers parsed. [718M] Kmers parsed. [719M] Kmers parsed. [719M] Kmers parsed. [720M] Kmers parsed. [721M] Kmers parsed. [722M] Kmers parsed. [722M] Kmers parsed. [723M] Kmers parsed. [724M] Kmers parsed. [724M] Kmers parsed. [725M] Kmers parsed. [726M] Kmers parsed. [727M] Kmers parsed. [727M] Kmers parsed. [728M] Kmers parsed. [729M] Kmers parsed. [730M] Kmers parsed. [730M] Kmers parsed. [731M] Kmers parsed. [732M] Kmers parsed. [732M] Kmers parsed. [733M] Kmers parsed. [734M] Kmers parsed. [734M] Kmers parsed. [735M] Kmers parsed. [736M] Kmers parsed. [736M] Kmers parsed. [737M] Kmers parsed. [738M] Kmers parsed. [739M] Kmers parsed. [739M] Kmers parsed. [740M] Kmers parsed. [741M] Kmers parsed. [742M] Kmers parsed. [742M] Kmers parsed. [743M] Kmers parsed. [744M] Kmers parsed. [745M] Kmers parsed. [745M] Kmers parsed. [746M] Kmers parsed. [747M] Kmers parsed. [747M] Kmers parsed. [748M] Kmers parsed. [749M] Kmers parsed. [750M] Kmers parsed. [750M] Kmers parsed. [751M] Kmers parsed. [752M] Kmers parsed. [752M] Kmers parsed. [753M] Kmers parsed. [754M] Kmers parsed. [754M] Kmers parsed. [755M] Kmers parsed. [756M] Kmers parsed. [756M] Kmers parsed. [757M] Kmers parsed. [758M] Kmers parsed. [758M] Kmers parsed. [759M] Kmers parsed. [760M] Kmers parsed. [761M] Kmers parsed. [761M] Kmers parsed. [762M] Kmers parsed. [763M] Kmers parsed. [763M] Kmers parsed. [764M] Kmers parsed. [765M] Kmers parsed. [766M] Kmers parsed. [766M] Kmers parsed. [767M] Kmers parsed. [768M] Kmers parsed. [768M] Kmers parsed. [769M] Kmers parsed. [770M] Kmers parsed. [770M] Kmers parsed. [771M] Kmers parsed. [772M] Kmers parsed. [773M] Kmers parsed. [773M] Kmers parsed. [774M] Kmers parsed. [775M] Kmers parsed. [775M] Kmers parsed. [776M] Kmers parsed. [777M] Kmers parsed. [777M] Kmers parsed. [778M] Kmers parsed. [779M] Kmers parsed. [780M] Kmers parsed. [780M] Kmers parsed. [781M] Kmers parsed. [782M] Kmers parsed. [782M] Kmers parsed. [783M] Kmers parsed. [784M] Kmers parsed. [784M] Kmers parsed. [785M] Kmers parsed. [786M] Kmers parsed. [786M] Kmers parsed. [787M] Kmers parsed. [788M] Kmers parsed. [788M] Kmers parsed. [789M] Kmers parsed. [790M] Kmers parsed. [790M] Kmers parsed. [791M] Kmers parsed. [791M] Kmers parsed. [792M] Kmers parsed. [793M] Kmers parsed. [794M] Kmers parsed. [794M] Kmers parsed. [795M] Kmers parsed. [796M] Kmers parsed. [796M] Kmers parsed. [797M] Kmers parsed. [798M] Kmers parsed. [798M] Kmers parsed. [799M] Kmers parsed. [800M] Kmers parsed. [800M] Kmers parsed. [801M] Kmers parsed. [802M] Kmers parsed. [803M] Kmers parsed. [803M] Kmers parsed. [804M] Kmers parsed. [805M] Kmers parsed. [805M] Kmers parsed. [806M] Kmers parsed. [807M] Kmers parsed. [807M] Kmers parsed. [808M] Kmers parsed. [809M] Kmers parsed. [810M] Kmers parsed. [810M] Kmers parsed. [811M] Kmers parsed. [812M] Kmers parsed. [813M] Kmers parsed. [813M] Kmers parsed. [814M] Kmers parsed. [815M] Kmers parsed. [816M] Kmers parsed. [816M] Kmers parsed. [817M] Kmers parsed. [818M] Kmers parsed. [818M] Kmers parsed. [819M] Kmers parsed. [820M] Kmers parsed. [820M] Kmers parsed. [821M] Kmers parsed. [822M] Kmers parsed. [822M] Kmers parsed. [823M] Kmers parsed. [824M] Kmers parsed. [824M] Kmers parsed. [825M] Kmers parsed. [825M] Kmers parsed. [826M] Kmers parsed. [827M] Kmers parsed. [827M] Kmers parsed. [828M] Kmers parsed. [829M] Kmers parsed. [829M] Kmers parsed. [830M] Kmers parsed. [831M] Kmers parsed. [831M] Kmers parsed. [832M] Kmers parsed. [832M] Kmers parsed. [833M] Kmers parsed. [833M] Kmers parsed. [834M] Kmers parsed. [835M] Kmers parsed. [835M] Kmers parsed. [836M] Kmers parsed. [837M] Kmers parsed. [838M] Kmers parsed. [838M] Kmers parsed. [839M] Kmers parsed. [839M] Kmers parsed. [840M] Kmers parsed. [841M] Kmers parsed. [841M] Kmers parsed. [842M] Kmers parsed. [842M] Kmers parsed. [843M] Kmers parsed. [844M] Kmers parsed. [844M] Kmers parsed. [845M] Kmers parsed. [845M] Kmers parsed. [846M] Kmers parsed. [847M] Kmers parsed. [847M] Kmers parsed. [848M] Kmers parsed. [848M] Kmers parsed. [849M] Kmers parsed. [850M] Kmers parsed. [850M] Kmers parsed. [851M] Kmers parsed. [852M] Kmers parsed. [852M] Kmers parsed. [853M] Kmers parsed. [853M] Kmers parsed. [854M] Kmers parsed. [855M] Kmers parsed. [855M] Kmers parsed. [856M] Kmers parsed. [857M] Kmers parsed. [857M] Kmers parsed. [858M] Kmers parsed. [859M] Kmers parsed. [860M] Kmers parsed. [860M] Kmers parsed. [861M] Kmers parsed. [862M] Kmers parsed. [862M] Kmers parsed. [863M] Kmers parsed. [863M] Kmers parsed. [864M] Kmers parsed. [865M] Kmers parsed. [865M] Kmers parsed. [866M] Kmers parsed. [867M] Kmers parsed. [867M] Kmers parsed. [868M] Kmers parsed. [869M] Kmers parsed. [869M] Kmers parsed. [870M] Kmers parsed. [871M] Kmers parsed. [871M] Kmers parsed. [872M] Kmers parsed. [873M] Kmers parsed. [874M] Kmers parsed. [874M] Kmers parsed. [875M] Kmers parsed. [876M] Kmers parsed. [876M] Kmers parsed. [877M] Kmers parsed. [877M] Kmers parsed. [878M] Kmers parsed. [879M] Kmers parsed. [879M] Kmers parsed. [880M] Kmers parsed. [880M] Kmers parsed. [881M] Kmers parsed. [882M] Kmers parsed. [883M] Kmers parsed. [883M] Kmers parsed. [884M] Kmers parsed. [885M] Kmers parsed. [885M] Kmers parsed. [886M] Kmers parsed. [887M] Kmers parsed. [887M] Kmers parsed. [888M] Kmers parsed. [888M] Kmers parsed. [889M] Kmers parsed. [890M] Kmers parsed. [891M] Kmers parsed. [891M] Kmers parsed. [892M] Kmers parsed. [893M] Kmers parsed. [893M] Kmers parsed. [894M] Kmers parsed. [895M] Kmers parsed. [895M] Kmers parsed. [896M] Kmers parsed. [896M] Kmers parsed. [897M] Kmers parsed. [898M] Kmers parsed. [898M] Kmers parsed. [899M] Kmers parsed. [899M] Kmers parsed. [900M] Kmers parsed. [901M] Kmers parsed. [901M] Kmers parsed. [902M] Kmers parsed. [903M] Kmers parsed. [904M] Kmers parsed. [904M] Kmers parsed. [905M] Kmers parsed. [905M] Kmers parsed. [906M] Kmers parsed. [907M] Kmers parsed. [907M] Kmers parsed. [908M] Kmers parsed. [908M] Kmers parsed. [909M] Kmers parsed. [909M] Kmers parsed. [910M] Kmers parsed. [911M] Kmers parsed. [911M] Kmers parsed. [912M] Kmers parsed. [913M] Kmers parsed. [913M] Kmers parsed. [914M] Kmers parsed. [915M] Kmers parsed. [915M] Kmers parsed. [916M] Kmers parsed. [916M] Kmers parsed. [917M] Kmers parsed. [917M] Kmers parsed. [918M] Kmers parsed. [918M] Kmers parsed. [919M] Kmers parsed. [920M] Kmers parsed. [920M] Kmers parsed. [921M] Kmers parsed. [921M] Kmers parsed. [922M] Kmers parsed. [923M] Kmers parsed. [923M] Kmers parsed. [924M] Kmers parsed. [925M] Kmers parsed. [925M] Kmers parsed. [926M] Kmers parsed. [926M] Kmers parsed. [927M] Kmers parsed. [928M] Kmers parsed. [929M] Kmers parsed. [929M] Kmers parsed. [930M] Kmers parsed. [930M] Kmers parsed. [931M] Kmers parsed. [932M] Kmers parsed. [932M] Kmers parsed. [933M] Kmers parsed. [934M] Kmers parsed. [934M] Kmers parsed. [935M] Kmers parsed. [936M] Kmers parsed. [936M] Kmers parsed. [937M] Kmers parsed. [937M] Kmers parsed. [938M] Kmers parsed. [939M] Kmers parsed. [939M] Kmers parsed. [940M] Kmers parsed. [941M] Kmers parsed. [941M] Kmers parsed. [942M] Kmers parsed. [943M] Kmers parsed. [943M] Kmers parsed. [944M] Kmers parsed. [945M] Kmers parsed. 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[975M] Kmers parsed. [976M] Kmers parsed. [977M] Kmers parsed. [977M] Kmers parsed. [978M] Kmers parsed. [979M] Kmers parsed. [979M] Kmers parsed. [980M] Kmers parsed. [981M] Kmers parsed. [981M] Kmers parsed. [982M] Kmers parsed. [982M] Kmers parsed. [983M] Kmers parsed. [983M] Kmers parsed. [984M] Kmers parsed. [985M] Kmers parsed. [985M] Kmers parsed. [986M] Kmers parsed. [986M] Kmers parsed. [987M] Kmers parsed. [988M] Kmers parsed. [989M] Kmers parsed. [989M] Kmers parsed. [990M] Kmers parsed. [990M] Kmers parsed. [990M] Kmers parsed. [991M] Kmers parsed. [991M] Kmers parsed. [992M] Kmers parsed. [992M] Kmers parsed. [993M] Kmers parsed. [994M] Kmers parsed. [995M] Kmers parsed. [995M] Kmers parsed. [996M] Kmers parsed. [997M] Kmers parsed. [997M] Kmers parsed. [998M] Kmers parsed. [998M] Kmers parsed. [999M] Kmers parsed. [1000M] Kmers parsed. [1000M] Kmers parsed. [1001M] Kmers parsed. [1001M] Kmers parsed. [1002M] Kmers parsed. [1003M] Kmers parsed. [1003M] Kmers parsed. [1004M] Kmers parsed. [1005M] Kmers parsed. [1005M] Kmers parsed. [1006M] Kmers parsed. [1007M] Kmers parsed. [1007M] Kmers parsed. [1008M] Kmers parsed. [1009M] Kmers parsed. [1009M] Kmers parsed. [1010M] Kmers parsed. [1011M] Kmers parsed. [1011M] Kmers parsed. [1012M] Kmers parsed. [1013M] Kmers parsed. [1013M] Kmers parsed. [1014M] Kmers parsed. [1014M] Kmers parsed. [1015M] Kmers parsed. [1016M] Kmers parsed. [1016M] Kmers parsed. [1017M] Kmers parsed. [1018M] Kmers parsed. [1018M] Kmers parsed. [1019M] Kmers parsed. [1019M] Kmers parsed. [1020M] Kmers parsed. [1020M] Kmers parsed. [1021M] Kmers parsed. [1022M] Kmers parsed. [1022M] Kmers parsed. [1023M] Kmers parsed. [1023M] Kmers parsed. [1024M] Kmers parsed. [1025M] Kmers parsed. [1025M] Kmers parsed. [1026M] Kmers parsed. [1027M] Kmers parsed. [1027M] Kmers parsed. [1028M] Kmers parsed. [1029M] Kmers parsed. [1029M] Kmers parsed. [1030M] Kmers parsed. [1031M] Kmers parsed. [1031M] Kmers parsed. [1032M] Kmers parsed. [1032M] Kmers parsed. [1033M] Kmers parsed. [1034M] Kmers parsed. [1034M] Kmers parsed. [1035M] Kmers parsed. [1036M] Kmers parsed. [1036M] Kmers parsed. [1037M] Kmers parsed. [1038M] Kmers parsed. [1038M] Kmers parsed. [1039M] Kmers parsed. [1039M] Kmers parsed. [1040M] Kmers parsed. [1041M] Kmers parsed. [1042M] Kmers parsed. [1042M] Kmers parsed. [1043M] Kmers parsed. [1043M] Kmers parsed. [1044M] Kmers parsed. [1045M] Kmers parsed. [1046M] Kmers parsed. [1046M] Kmers parsed. [1047M] Kmers parsed. [1048M] Kmers parsed. [1048M] Kmers parsed. [1049M] Kmers parsed. [1049M] Kmers parsed. [1050M] Kmers parsed. [1051M] Kmers parsed. [1051M] Kmers parsed. [1052M] Kmers parsed. [1053M] Kmers parsed. [1053M] Kmers parsed. [1054M] Kmers parsed. [1055M] Kmers parsed. [1055M] Kmers parsed. [1056M] Kmers parsed. [1057M] Kmers parsed. [1057M] Kmers parsed. [1058M] Kmers parsed. [1059M] Kmers parsed. [1059M] Kmers parsed. [1060M] Kmers parsed. [1061M] Kmers parsed. [1061M] Kmers parsed. [1062M] Kmers parsed. [1062M] Kmers parsed. [1063M] Kmers parsed. [1064M] Kmers parsed. [1064M] Kmers parsed. [1065M] Kmers parsed. [1066M] Kmers parsed. [1066M] Kmers parsed. [1067M] Kmers parsed. [1068M] Kmers parsed. [1068M] Kmers parsed. [1069M] Kmers parsed. [1070M] Kmers parsed. [1070M] Kmers parsed. [1071M] Kmers parsed. [1071M] Kmers parsed. [1072M] Kmers parsed. [1073M] Kmers parsed. [1073M] Kmers parsed. [1074M] Kmers parsed. [1075M] Kmers parsed. [1076M] Kmers parsed. [1076M] Kmers parsed. [1077M] Kmers parsed. [1078M] Kmers parsed. [1078M] Kmers parsed. [1079M] Kmers parsed. [1080M] Kmers parsed. [1080M] Kmers parsed. [1081M] Kmers parsed. [1082M] Kmers parsed. [1082M] Kmers parsed. [1083M] Kmers parsed. [1083M] Kmers parsed. [1084M] Kmers parsed. [1085M] Kmers parsed. [1085M] Kmers parsed. [1086M] Kmers parsed. [1087M] Kmers parsed. [1087M] Kmers parsed. [1088M] Kmers parsed. [1089M] Kmers parsed. [1089M] Kmers parsed. [1090M] Kmers parsed. [1091M] Kmers parsed. [1091M] Kmers parsed. [1092M] Kmers parsed. [1093M] Kmers parsed. [1093M] Kmers parsed. [1094M] Kmers parsed. [1095M] Kmers parsed. [1095M] Kmers parsed. [1096M] Kmers parsed. [1097M] Kmers parsed. [1097M] Kmers parsed. [1098M] Kmers parsed. [1099M] Kmers parsed. [1099M] Kmers parsed. [1100M] Kmers parsed. [1101M] Kmers parsed. [1101M] Kmers parsed. [1102M] Kmers parsed. [1103M] Kmers parsed. [1103M] Kmers parsed. [1104M] Kmers parsed. [1105M] Kmers parsed. [1105M] Kmers parsed. [1106M] Kmers parsed. [1106M] Kmers parsed. [1107M] Kmers parsed. [1108M] Kmers parsed. [1108M] Kmers parsed. [1109M] Kmers parsed. [1109M] Kmers parsed. [1110M] Kmers parsed. [1111M] Kmers parsed. [1111M] Kmers parsed. [1112M] Kmers parsed. [1112M] Kmers parsed. [1113M] Kmers parsed. [1113M] Kmers parsed. [1114M] Kmers parsed. [1115M] Kmers parsed. [1115M] Kmers parsed. [1116M] Kmers parsed. [1116M] Kmers parsed. [1117M] Kmers parsed. [1118M] Kmers parsed. [1118M] Kmers parsed. [1119M] Kmers parsed. [1120M] Kmers parsed. [1120M] Kmers parsed. [1121M] Kmers parsed. [1122M] Kmers parsed. [1122M] Kmers parsed. [1123M] Kmers parsed. [1124M] Kmers parsed. [1124M] Kmers parsed. [1125M] Kmers parsed. [1126M] Kmers parsed. [1126M] Kmers parsed. [1127M] Kmers parsed. [1128M] Kmers parsed. [1128M] Kmers parsed. [1129M] Kmers parsed. [1130M] Kmers parsed. [1130M] Kmers parsed. [1131M] Kmers parsed. [1132M] Kmers parsed. [1132M] Kmers parsed. [1133M] Kmers parsed. [1134M] Kmers parsed. [1134M] Kmers parsed. [1135M] Kmers parsed. [1136M] Kmers parsed. [1136M] Kmers parsed. [1137M] Kmers parsed. [1138M] Kmers parsed. [1138M] Kmers parsed. [1139M] Kmers parsed. [1140M] Kmers parsed. [1140M] Kmers parsed. [1141M] Kmers parsed. [1142M] Kmers parsed. [1142M] Kmers parsed. [1143M] Kmers parsed. [1144M] Kmers parsed. [1144M] Kmers parsed. [1145M] Kmers parsed. [1146M] Kmers parsed. [1146M] Kmers parsed. [1147M] Kmers parsed. [1147M] Kmers parsed. [1148M] Kmers parsed. [1149M] Kmers parsed. [1149M] Kmers parsed. [1150M] Kmers parsed. [1151M] Kmers parsed. [1151M] Kmers parsed. [1152M] Kmers parsed. [1153M] Kmers parsed. [1153M] Kmers parsed. [1154M] Kmers parsed. [1155M] Kmers parsed. [1155M] Kmers parsed. [1156M] Kmers parsed. [1157M] Kmers parsed. [1157M] Kmers parsed. [1158M] Kmers parsed. [1159M] Kmers parsed. [1159M] Kmers parsed. [1160M] Kmers parsed. [1161M] Kmers parsed. [1161M] Kmers parsed. [1162M] Kmers parsed. [1163M] Kmers parsed. [1163M] Kmers parsed. [1164M] Kmers parsed. [1165M] Kmers parsed. [1165M] Kmers parsed. [1166M] Kmers parsed. [1167M] Kmers parsed. [1167M] Kmers parsed. [1168M] Kmers parsed. [1168M] Kmers parsed. [1169M] Kmers parsed. [1170M] Kmers parsed. [1170M] Kmers parsed. [1171M] Kmers parsed. [1171M] Kmers parsed. [1172M] Kmers parsed. [1173M] Kmers parsed. [1173M] Kmers parsed. [1174M] Kmers parsed. [1175M] Kmers parsed. [1175M] Kmers parsed. [1176M] Kmers parsed. [1177M] Kmers parsed. [1177M] Kmers parsed. [1178M] Kmers parsed. [1179M] Kmers parsed. [1179M] Kmers parsed. [1180M] Kmers parsed. [1180M] Kmers parsed. [1181M] Kmers parsed. [1181M] Kmers parsed. [1182M] Kmers parsed. [1183M] Kmers parsed. [1183M] Kmers parsed. [1184M] Kmers parsed. [1185M] Kmers parsed. [1185M] Kmers parsed. [1186M] Kmers parsed. [1187M] Kmers parsed. [1187M] Kmers parsed. [1188M] Kmers parsed. [1189M] Kmers parsed. [1189M] Kmers parsed. [1190M] Kmers parsed. [1191M] Kmers parsed. [1191M] Kmers parsed. [1192M] Kmers parsed. [1192M] Kmers parsed. [1193M] Kmers parsed. [1194M] Kmers parsed. [1194M] Kmers parsed. [1195M] Kmers parsed. [1196M] Kmers parsed. [1196M] Kmers parsed. [1197M] Kmers parsed. [1198M] Kmers parsed. [1198M] Kmers parsed. [1199M] Kmers parsed. [1199M] Kmers parsed. [1200M] Kmers parsed. [1201M] Kmers parsed. [1202M] Kmers parsed. [1202M] Kmers parsed. [1203M] Kmers parsed. [1203M] Kmers parsed. [1204M] Kmers parsed. [1205M] Kmers parsed. [1205M] Kmers parsed. [1206M] Kmers parsed. [1207M] Kmers parsed. [1207M] Kmers parsed. [1208M] Kmers parsed. [1209M] Kmers parsed. [1209M] Kmers parsed. [1210M] Kmers parsed. [1211M] Kmers parsed. [1212M] Kmers parsed. [1212M] Kmers parsed. [1213M] Kmers parsed. [1213M] Kmers parsed. [1214M] Kmers parsed. [1215M] Kmers parsed. [1215M] Kmers parsed. [1216M] Kmers parsed. [1217M] Kmers parsed. [1217M] Kmers parsed. [1218M] Kmers parsed. [1218M] Kmers parsed. [1219M] Kmers parsed. [1219M] Kmers parsed. [1220M] Kmers parsed. [1220M] Kmers parsed. [1221M] Kmers parsed. [1222M] Kmers parsed. [1222M] Kmers parsed. [1223M] Kmers parsed. [1224M] Kmers parsed. [1224M] Kmers parsed. [1225M] Kmers parsed. [1225M] Kmers parsed. [1226M] Kmers parsed. [1227M] Kmers parsed. [1227M] Kmers parsed. [1228M] Kmers parsed. [1228M] Kmers parsed. [1229M] Kmers parsed. [1229M] Kmers parsed. [1230M] Kmers parsed. [1231M] Kmers parsed. [1231M] Kmers parsed. [1232M] Kmers parsed. [1233M] Kmers parsed. [1233M] Kmers parsed. [1234M] Kmers parsed. [1235M] Kmers parsed. 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[1321M] Kmers parsed. [1322M] Kmers parsed. done parsing 1322562903 Kmers, 1322562903 added, taking 2766 seconds. TIMING KMER_DB_BUILDING 2766 s. Pruning kmers (min_kmer_count=1 min_any_entropy=0 min_ratio_non_error=0.05) Pruned 16951521 kmers from catalog. Pruning time: 2902 seconds = 48.3667 minutes. TIMING PRUNING 2902 s. -populating the kmer seed candidate list. Kcounter hash size: 1322562903 terminate called after throwing an instance of 'std::bad_alloc' what(): std::bad_alloc Aborted (core dumped) Error, cmd: /home/shared/trinityrnaseq-Trinity-v2.6.5/Inchworm/bin//inchworm --kmers jellyfish.kmers.fa --run_inchworm -K 25 -L 25 --monitor 1 --DS --num_threads 6 --PARALLEL_IWORM > /media/sam/4TB_toshiba/montipora/20180402_trinity/inchworm.K25.L25.DS.fa.tmp 2>tmp.12593.stderr died with ret 34304 at /home/shared/trinityrnaseq-Trinity-v2.6.5/PerlLib/Pipeliner.pm line 166. Pipeliner::run(Pipeliner=HASH(0x183bc60)) called at /home/shared/trinityrnaseq-Trinity-v2.6.5/Trinity line 2406 eval {...} called at /home/shared/trinityrnaseq-Trinity-v2.6.5/Trinity line 2401 main::run_inchworm("/media/sam/4TB_toshiba/montipora/20180402_trinity/inchworm.K2"..., "/media/sam/4TB_toshiba/montipora/20180402_trinity/both.fa", undef, "") called at /home/shared/trinityrnaseq-Trinity-v2.6.5/Trinity line 1605 main::run_Trinity() called at /home/shared/trinityrnaseq-Trinity-v2.6.5/Trinity line 1314 eval {...} called at /home/shared/trinityrnaseq-Trinity-v2.6.5/Trinity line 1313 If it indicates bad_alloc(), then Inchworm ran out of memory. You'll need to either reduce the size of your data set or run Trinity on a server with more memory available. ** The inchworm process failed.