Roberts Mentoring Plan Week 1 (June 20) Themes: Orientation Goals Learn what others in the lab are working on Understand logistics Tasks Attend lab meeting Set-up lab notebook Get on lab calendar and lab slack Schedule safety sessions Review Lab handbook, specifically onboarding. Week 2 Themes: Design Experiment Goals: Understand big picture of what research will look like Tasks Read select papers Meet with Mentor to go over details Write up research plan in notebook Week 3 Theme: Start experiment Goals: Get mussels in experimental system Tasks Aquire mussels Set up mussels in system Read about sample collection Write up sample collection plan in notebook. Week 4 Theme: Environmental stressor Goal: Expose mussel to stressors Tasks Expose mussels to conditions determined in Week 2 Sample mussels after 24 hours. Week 5 Theme: Sampling Goal: Complete sampling Tasks Sample mussels Start RNA extraction Plan out cDNA synthesis protocol Week 6 Theme: Sample Processing Goal: Prep for qPCR Tasks Finish RNA extractions Perform cDNA synthesis Plan out qPCR protocol Week 7 Theme: Gene Expression Goal: Run qPCR Tasks Run qPCR Establish initial findings Write Abstract Week 8 (Abstract DUE) Theme: Final Stretch Goal: Developing final products Tasks: Finish up any final analysis Start Poster Read relevant papers with similar efforts Week 9 (Poster Presentation) Theme: Presenting Goal: Have clear picture of meaning of results Tasks: Give practice presentation