---
title: "03-kallisto"
author: "Steven Roberts"
date: "`r format(Sys.time(), '%d %B, %Y')`"  
output: 
  github_document:
    toc: true
    toc_depth: 3
    number_sections: true
    html_preview: true
  html_document:
    theme: readable
    highlight: zenburn
    toc: true
    toc_float: true
    number_sections: true
    code_folding: show
    code_download: true
editor_options: 
  markdown: 
    wrap: sentence
---

```{r setup, include=FALSE}
library(knitr)
library(tidyverse)
library(kableExtra)
library(DT)
library(Biostrings)
library(tm)
library(pheatmap)
library(DESeq2)
knitr::opts_chunk$set(
  echo = TRUE,         # Display code chunks
  eval = FALSE,         # Evaluate code chunks
  warning = FALSE,     # Hide warnings
  message = FALSE,     # Hide messages
  fig.width = 6,       # Set plot width in inches
  fig.height = 4,      # Set plot height in inches
  fig.align = "center", # Align plots to the center
  comment = ""         # Prevents appending '##' to beginning of lines in code output
)
```




Reads

```{r, engine='bash', eval=TRUE}
ls ../data/
```

#laby 18s


```{bash}
head ../data/*fasta
```


```{bash}
/home/shared/kallisto/kallisto index \
-i ../output/03-kallisto/18slaby.index \
../data/laby18s-sequence.fasta
```


```{bash}
find ../data/*_L001_R1_001.fastq.gz | xargs basename -s _L001_R1_001.fastq.gz \
| xargs -I{} sh -c '/home/shared/kallisto/kallisto quant \
-i ../output/03-kallisto/18slaby.index \
-o ../output/03-kallisto/{} \
-t 20 \
--fr-stranded ../data/{}_L001_R1_001.fastq.gz \
--rf-stranded ../data/{}_L001_R2_001.fastq.gz \
> ../output/03-kallisto/{}_kal.stdout 2> ../output/03-kallisto/{}_kal.stderr'
```


```{r, engine='bash'}
eval "$(/opt/anaconda/anaconda3/bin/conda shell.bash hook)"
conda activate
which multiqc


multiqc ../output/03-kallisto/ \
-o ../output/03-kallisto/
```

```{bash}

cat ../data/*fasta | grep ">"
```


```{bash}
perl /home/shared/trinityrnaseq-v2.12.0/util/abundance_estimates_to_matrix.pl \
--est_method kallisto \
    --gene_trans_map none \
    --out_prefix ../output/03-kallisto/comb \
    --name_sample_by_basedir \
    ../output/03-kallisto/*/abundance.tsv \
```