• Examine genetic differences over space, time, and host species in Alaskan Chionoecetes and Hematodinium

  • Changes in host, parasite, host/parasite interactions
  • Determine Hematodinium population structure
  • Measure Hematodinium gene flow
  • Better understand Hematodinium transmission dynamics

• Possible exploration of 2020-2021 mortality event

• Funding source: NPRB

• Rolling deadline (began Sept. 28th)

• Timeline:

  • 1-6 months: Proposal peer review
  • Dates TBD: Approved at board meeting
  • 1 year: NRPB suggested project start date

• Co-funding partners: BSFRF, Oil Spill Recovery Institute, Pollock Conservation Cooperative Research Center

• Goal research category: Fishes and Invertebrates
  • Project cap: $600,000
  • Total goal: $1,000,000
• Relevant general topics:
  • Estimation of life history parameters that impact stock assessments
  • Ecology and physiology of forage species
  • Characterization of cross-species interactions
  • Analyses of survey design and data (depending on methods)
    • qPCR vs. cPCR vs. histology

• Iron out specifics of methods
• Ensure samples can be extracted and amplified
• Apply for NPRB!

Samples were shipped from Pam Jensen to the Roberts Lab upon her retirement from NOAA.

Uses Pam’s data tables - which have not yet been cross-referenced with the physical samples - as the data source.

Total samples: 27,387

• Majority: hemolymph samples in deep well plates
• Minority: tissue samples in tubes
• All: Preserved in 100% EtOH