FastQCFastQC Report
Thu 14 Dec 2023
F5-T127G_S137_L099_R1_cmb.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameF5-T127G_S137_L099_R1_cmb.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418975
Total Bases156.2 Mbp
Sequences flagged as poor quality0
Sequence length20-95
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGA28080.11608222490931076No Hit
AAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCT25040.10351491850887255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)