FastQCFastQC Report
Thu 14 Dec 2023
C7-T051F_S150_L099_R1_cmb.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7-T051F_S150_L099_R1_cmb.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942703
Total Bases63.1 Mbp
Sequences flagged as poor quality0
Sequence length20-95
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGA40340.4279184430303075No Hit
AAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCT34930.37053027305524644No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG34880.369999883314257No Hit
TTTGCAGTTATATTTATTGCGGAGTACTCTAGTATTCTTCTCAGAAGGGT27920.2961696313685222No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG22760.2414334100984085No Hit
ATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGT18620.19751713954448005No Hit
CTCTTATACGATTTTGATGGGAGATCAGTG16640.1765137058012969No Hit
ACTCTTTGACTAATGGTGAGGTTAGTCCGACTATGGCGTGGGGAGTTTTC16430.1742860688891411No Hit
TCTGTTCGCCCTTTAAAATCTAACATGAGCTG15920.1688760935310485No Hit
AGTTTTCCAGTCAACGGCACTTTGGTTTTGAGGCTTGCATTTGGTACTGA14800.15699536333288427No Hit
ATCGCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCAT14750.1564649735918948No Hit
AATGGTTATGGGGTTAAGAGTTGCGTTTGTCTGCATTGTGTCTTTTTTGT14660.15551027205811374No Hit
AGTTTTTGGCTCTTATACGATTTTGATGGGAGATCAGTG14470.15349479104235375No Hit
ACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTT13690.14522071108291795No Hit
CCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGT12910.13694663112348215No Hit
ATGAACCAAACGTTCGGTTAAGGTGCCAAACACGGACGCTCATCAGATAC12750.13524938395231584No Hit
ATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGC12460.13217312345457688No Hit
TAAAATTTTTTGGGTTAAGCTGAAAAATACTAAAATTGATGTT12430.1318548896099832No Hit
TTGATGAGTCTCGTTCCAACAGGTTGCCCGTTAATCCTTGTGCCTTTTAT12010.12739961578567163No Hit
ACGTGTGCTCTTCCGATCTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.12506590092531794No Hit
CAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTC11750.12464158913252636No Hit
TTTATGTGGTGAGATAGACTGAGCAGGTACCCGAGACCTTTATCCAGGAA11210.11891337992984005No Hit
AAGCTTCTAATGGTGGGTCTGTTCGCCCTTTAAAATCTAACATGAGCTG11120.11795867839605899No Hit
AACTGTGGTGTTTGGGACGTCTATTGGCGCGATGTCCGGGTGCCTAGGTC11110.11785260044786108No Hit
CTAGCTACTCTAGGGATAACAGCGCAATTTCTCCCGAAAGATGGTATTGG11050.11721613275867374No Hit
AAAGAGCGCGTTTATTAGATCAAAACCAGTCGGGTCCGCAAGGGCCCGTC10960.11626143122489267No Hit
TCCGCTGTGTCTTTTGGGTCTCTAATATATTTTAAGTTCCTTCTATGAGA10800.11456418405372637No Hit
TCTCTAATATATTTTAAGTTCCTTCTATGAGAGGCTCTAGTAAGCCAGCG10740.113927716364539No Hit
TTAAGCTGAAAAATACTAAAATTGATGTT10690.11339732662354951No Hit
CAAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCA10650.11297301483075793No Hit
TCTTTCACTATTATATAAATCTAACTCGTATTTCCTAACTTTATATGTGT10640.11286693688256004No Hit
AATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACATCC10500.11138184560778952No Hit
CGCCGGCGACGTATCTTTCAAATGTCTGCCCTATCAACTTTCGATGGTAC10290.10915420869563373No Hit
ATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTAATGGTGGGTCT10260.10883597485104005No Hit
AGCTGGTAAAGTAATTCTAACTATATGCAGGAGAGAGTTAGTAGTTAGCT10220.10841166305824847No Hit
TAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTA10030.10639618204248846No Hit
AAGAGCTCAGCACCGAATCCCGCAGCCTTGCGCTGCAGGGAACTGTGGTG9880.10480501281952004No Hit
TTTTAAGCAAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG9860.10459285692312424No Hit
CCCCGTAATTGGAATGAGTACACTTTAAACCCTTTAACGAGGATCTATTG9720.10310776564835372No Hit
ATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCT9600.10183483026997898No Hit
CAGAGATTTAATTAAACTAAAGAATAGCGGCTAGATACTATTCGCGCTTT9480.10056189489160425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)