FastQCFastQC Report
Sat 4 Mar 2023
SRR12227929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12227929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4005096
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGTT93820.2342515635080907TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTTT76990.19223009885405992TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT48730.12166999242964463TruSeq Adapter, Index 21 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43530.10868653335650383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)