FastQCFastQC Report
Tue 9 Apr 2024
3291_5903_10012_H94MGADXX_HK_CF70_GCCAAT_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3291_5903_10012_H94MGADXX_HK_CF70_GCCAAT_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11952919
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA326720.273339089807268No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT289870.24250980032576144No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA179440.1501223257682914No Hit
CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG176020.14726109998737547No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC174530.1460145425565086TruSeq Adapter, Index 6 (100% over 50bp)
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC153690.12857947083888044No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT152210.1273412795652677No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT142910.11956075331891734No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG135840.11364588014024023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)