FastQCFastQC Report
Tue 9 Apr 2024
3291_5903_10010_H94MGADXX_HK_CF2_TGACCA_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3291_5903_10010_H94MGADXX_HK_CF2_TGACCA_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23172864
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT1567180.6762996580828334No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA1379220.5951875434991548No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA941920.40647543609628917No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT773930.33398116003270034No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG668610.2885314478175853No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT596000.25719738397463515No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC476410.20558960687811398No Hit
TCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGA430310.1856956481512169No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT259380.1119326467371491No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT234410.10115711204277554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)