FastQCFastQC Report
Tue 9 Apr 2024
3291_5903_10010_H94MGADXX_HK_CF2_TGACCA_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3291_5903_10010_H94MGADXX_HK_CF2_TGACCA_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23172864
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA1558910.672730828610568No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT1396210.6025193950993715No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA772540.3333813204962494No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT743930.32103498298699723No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT660050.2848374719672113No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC623440.26903882057910494No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG615870.2657720685712392No Hit
TCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGA479070.206737501242833No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC329980.1423993167180371TruSeq Adapter, Index 4 (100% over 50bp)
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT258850.11170393094267503No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT245970.10614570559771981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)