FastQCFastQC Report
Tue 9 Apr 2024
3291_5903_10008_H94MGADXX_V_CF34_CGATGT_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3291_5903_10008_H94MGADXX_V_CF34_CGATGT_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9752597
Total Bases985 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA287260.2945471857393472No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT281100.2882309194156182No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA250550.2569059297743975No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG207270.21252800664274346No Hit
CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG179700.18425861337241764No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT178600.18313070867175174No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC151400.1552407015280135No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT104350.10699714137680455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)