FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-53-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25726329
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG18483827.1847872271243975Illumina RNA PCR Primer (100% over 37bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG15491766.021753045294569Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG14619935.68286676268503Illumina RNA PCR Primer (100% over 36bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA8945543.477192567971901Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA5239212.0365167529343187Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG4582951.7814240034013402Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG4133571.6067469245223442No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT2266970.8811867406344683Illumina RNA PCR Primer (100% over 50bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1569410.6100403986903845No Hit
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1528740.5942316915872451Illumina RNA PCR Primer (100% over 37bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1401710.5448542619508598Illumina RNA PCR Primer (100% over 45bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1354060.5263323811181921Illumina RNA PCR Primer (100% over 37bp)
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1324800.5149588190371039Illumina RNA PCR Primer (100% over 36bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1261790.49046640117212215Illumina Small RNA Adapter 1 (100% over 26bp)
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1095230.4257233902279645Illumina RNA PCR Primer (100% over 36bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC974130.378650992141164Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT970600.3772788570028782Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT960280.37326740243429213Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG948950.36886335395928427Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG938400.36476249681794864Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC878700.3415566985868835Illumina RNA PCR Primer (100% over 32bp)
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC844280.32817740922150224Illumina RNA PCR Primer (100% over 30bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT836970.3253359622354204Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA794110.3086759871569706Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC753390.29284784471192915Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT710740.27626949806946804Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT672540.26142089685629066Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC606830.23587897052859738Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT582390.22637897540686822Illumina RNA PCR Primer (100% over 31bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT547270.21272759125485802Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGTTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG527890.20519445273361778Illumina DpnII expression Sequencing Primer (96% over 29bp)
CTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA515000.2001840215912655Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG499150.19402301820831103Illumina Small RNA Adapter 1 (100% over 26bp)
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT486920.18926913357906602Illumina RNA PCR Primer (100% over 35bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT484820.18845284921917932No Hit
TCCACAGAATTATTTAAAAGTGATGCAAGAGATCGTCGGACTGTAGAACT476100.18506332559145924No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT470310.18281271299920016No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC465380.18089638828765658No Hit
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA447670.1740123901859453Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT429570.1669767964173979Illumina RNA PCR Primer (100% over 35bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT424440.1649827303382461Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG406160.15787716933885126Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG401570.15609300495224174Illumina RNA PCR Primer (100% over 34bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG377080.14657357448861047Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTG351360.13657603461418844Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA349800.13596965194684404Illumina DpnII expression Sequencing Primer (100% over 25bp)
GTAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG336370.13074931911194945No Hit
CCAGAATTGCCTGAAGCCGTGCAGGAAGATCGTCGGACTGTAGAACTCTG336250.13070267429138452Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG322060.12518692425957859Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC307410.11949236908227366Illumina RNA PCR Primer (100% over 32bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT291270.11321864071628719Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288480.11213414863815198No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC288470.11213026156977157Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCAGAATTGCCTGAAGCCGTGCAGGAAGATCGTCGGACTGTAGAACTCT288390.11209916502272828Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT286780.1114733470134818Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA280020.10884568878832265Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA279620.10869020605310613Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC278810.1083753535142927Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG271520.10554168066497166Illumina Small RNA Adapter 1 (100% over 26bp)
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC270330.10507911952770253Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC266850.10372641973131884Illumina RNA PCR Primer (100% over 30bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC264890.1029645543287579No Hit
TGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT263540.10243980009740215Illumina RNA PCR Primer (100% over 31bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG257720.10017752630000183Illumina Small RNA Adapter 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)