FastQCFastQC Report
Wed 15 Nov 2023
sRNA-POR-79-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-79-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19762559
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4788022.422773285585131No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3989382.0186555799782813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG2655961.3439352666828217TruSeq Adapter, Index 22 (97% over 49bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2420091.2245833143369742No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTTTG2049421.0370215719533082TruSeq Adapter, Index 22 (97% over 46bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACACGTCTGAA1962110.9928420707055194Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1937520.9803993501044069No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAT1845530.9338517344843854TruSeq Adapter, Index 22 (97% over 41bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT1735250.8780492445335648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1498220.7581103236681039No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGGTTG1384800.7007189706555714TruSeq Adapter, Index 22 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTATG1351490.6838638660104696TruSeq Adapter, Index 22 (97% over 42bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1273320.6443092718913578No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1178470.5963144752660827No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC1086920.5499895028776385No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTTTG1022410.5173469690843174TruSeq Adapter, Index 22 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGGTTG832420.4212106337038639TruSeq Adapter, Index 22 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGGATG829710.4198393538002847TruSeq Adapter, Index 22 (97% over 45bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC647910.32784721857123866No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC634370.3209958791267872No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC634130.32087443736410853No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT597330.3022533670867219No Hit
GTTCAAGATAGTCGAACTCGACTCTGTTAGATCGGAAGAGCACACGTCTG579240.2930996942248218Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC571660.28926415855355575No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGTAGATCGGAAGAGCACACGTC549190.277894173522771No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGGATG542950.2747366876931272TruSeq Adapter, Index 22 (97% over 42bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG513730.2599511530870066No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA498520.25225478137724977No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCAGATCGGAAGAGCACACGTCTG471820.23874438527925457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA460350.2329404810379061TruSeq Adapter, Index 22 (100% over 37bp)
TTGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAT443420.2243737766956192TruSeq Adapter, Index 22 (97% over 41bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGAGATCGGAAGAGCACACGTCT428790.21697088924566904Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG424920.21501264082247648Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTTGAGCCCCTGACGAAGTAGGCTAGATCGGAAGAGCACACGTCTGA417110.21106072346197677Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC411310.2081258808639104Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA382610.19360347007692677TruSeq Adapter, Index 22 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTTTT374120.189307467722171TruSeq Adapter, Index 22 (97% over 46bp)
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGC369440.18693935334993814No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG367460.1859374588078396Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC366690.18554783315257908No Hit
GTTCAAGATAGTCGAACTCGACTCTGTAGATCGGAAGAGCACACGTCTGA366010.18520374815832302Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA355290.17977934942534518No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCGAGATCGGAAGAGCACACGTCT346320.17524046354523218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC337910.17098494177803594No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATT336440.17024111098162945TruSeq Adapter, Index 22 (97% over 48bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG314150.15896220727285368No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC297800.15068898719037346No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC297350.15046128388535107No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCCAGATCGGAAGAGCACACGTCT297290.15043092344468142Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGTTAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC295270.14940878860880313No Hit
TGAAAATCTTTTCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC288690.14607926028203128Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGTGCTTAAGCGTGAGTAGTTGTCCAGATCGGAAGAGCACACGTCTGAA287970.14571493499399546Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGGGTG280980.142177943655981TruSeq Adapter, Index 22 (97% over 42bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA270260.13675354492300315Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGGGTG269880.13656126213209535TruSeq Adapter, Index 22 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGGTTT263180.13317101292398417TruSeq Adapter, Index 22 (97% over 45bp)
CACCCCTTCTCCAAATACTAGACCTCTTAGATCGGAAGAGCACACGTCTG262590.1328724685907326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA256660.129871845037882No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTGTG256170.12962390143907981TruSeq Adapter, Index 22 (97% over 46bp)
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG252310.1276707130893322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT250990.12700278339459986No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTGTG245090.12401734006208405TruSeq Adapter, Index 22 (97% over 42bp)
GAGGTCCGGACGGTCGAGGGTTATCATCCTAGATCGGAAGAGCACACGTC230270.11651831121667999No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC226550.11463596389516156No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTAGATCGGAAGAGCACACG222580.11262711473751957No Hit
GATTCTATAGACACCCCTTCTCCAAATACTAGACCTCTTTAGATCGGAAG216410.10950504942199035No Hit
CACCCCTCTTCCACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCT206540.10451075693183255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTC204440.10344814150839474TruSeq Adapter, Index 22 (98% over 50bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT199960.10118122860506072No Hit
TGGGTTGAGTGAATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC198720.10055377949788791Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)