FastQCFastQC Report
Thu 5 Sep 2024
RNA-POC-57-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-57-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42513488
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTA739100.17385070827404234No Hit
AATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATGAGACA527380.12405004265940257No Hit
TTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGTTTGTT445010.10467501513872493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)